> Details

Workalemahu, Grefachew; Foerster, Martin; Kroegel, Claus

Expression of metalloproteinase‐7 (matrilysin) in human blood and bronchoalveolar Gamma/Delta T‐lymphocytes. Selective upregulation by the soluble non‐peptidic mycobacterial phosphoantigen (isopentenyl pyrophosphate)

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Expression of metalloproteinase‐7 (matrilysin) in human blood and bronchoalveolar Gamma/Delta T‐lymphocytes. Selective upregulation by the soluble non‐peptidic mycobacterial phosphoantigen (isopentenyl pyrophosphate)
  • Contributor: Workalemahu, Grefachew; Foerster, Martin; Kroegel, Claus
  • imprint: Wiley, 2006
  • Published in: Journal of Cellular Physiology
  • Language: English
  • DOI: 10.1002/jcp.20541
  • ISSN: 0021-9541; 1097-4652
  • Keywords: Cell Biology ; Clinical Biochemistry ; Physiology
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p>Human γδ T‐lymphocytes are believed to regulate local immune defense and enhance resistance against invading microbes, although their precise function remains unknown. Herein, we addressed the question whether γδ T‐lymphocytes mediate these processes via synthesis of MMP‐7, a protease closely associated with both epithelial repair and mucosal defense. Blood and bronchoalveolar γδ T‐lymphocytes were cultured in the absence and presence of isopentenyl pyrophosphate (IPP) or TGF‐β1/IL‐15 for 24 h, and assessed for the expression and synthesis of MMP‐1, MMP‐7, and MMP‐9. Resting human γδ T‐lymphocytes constitutively expressed MMP‐9 mRNA, a marginal or no MMP‐7 and MMP‐1 mRNA. In the presence of IPP (3 µg/ml), expression of MMP‐7 mRNA significantly increased, whereas TGF‐β1/IL‐15 had no effect. Further, quiescent γδ T‐lymphocytes obtained from bronchoalveolar lavage (BAL) fluid showed a weak or no MMP‐7 mRNA signal which was raised significantly following stimulation with IPP. In Western blot analysis, a 28‐kDa pro‐matrilysin could be detected both in cell lysates (2 days) and supernatants (5 days) with a four‐ to sevenfold increased signal following IPP‐stimulation of the γδ T‐lymphocytes. In conclusion, the data demonstrate for the first time that both human blood and BAL γδ T‐lymphocytes express MMP‐7 mRNA and synthesize MMP‐7‐protein. This unfolds a new perspective for the understanding of γδ T‐lymphocyte function. J. Cell. Physiol. 207: 67–74, 2006. © 2005 Wiley‐Liss, Inc.</jats:p>