Description:
<jats:p>The overall folding of an RNA molecule is reflected in its base pairing pattern. The identification of that pattern provides a first step towards the determination of the structure of an RNA molecule. We show that the application of heteronuclear NMR methods at 750 MHz to <jats:italic>E. coli</jats:italic> 5S rRNA (120 nucleotides) selectively labelled with <jats:sup>15</jats:sup>N in guanine and uridine allows observation of base pairing patterns for a larger RNA molecule. We also present evidence that the fold of the E‐domain of the 5S rRNA (nt 79–97) as a contiguous part of the 5S rRNA and as an isolated molecule is virtually the same.</jats:p>