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Onodera, Taishi; Sax, Nicolas; Sato, Takashi; Adachi, Yu; Kotaki, Ryutaro; Inoue, Takeshi; Shinnakasu, Ryo; Nakagawa, Takayuki; Fukushi, Shuetsu; Terooatea, Tommy; Yoshikawa, Mai; Tonouchi, Keisuke; Nagakura, Takaki; Moriyama, Saya; Matsumura, Takayuki; Isogawa, Masanori; Terahara, Kazutaka; Takano, Tomohiro; Sun, Lin; Nishiyama, Ayae; Omoto, Shinnya; Shinkai, Masaharu; Kurosaki, Tomohiro; Yamashita, Kazuo;

CD62L expression marks SARS-CoV-2 memory B cell subset with preference for neutralizing epitopes

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  • Media type: E-Article
  • Title: CD62L expression marks SARS-CoV-2 memory B cell subset with preference for neutralizing epitopes
  • Contributor: Onodera, Taishi; Sax, Nicolas; Sato, Takashi; Adachi, Yu; Kotaki, Ryutaro; Inoue, Takeshi; Shinnakasu, Ryo; Nakagawa, Takayuki; Fukushi, Shuetsu; Terooatea, Tommy; Yoshikawa, Mai; Tonouchi, Keisuke; Nagakura, Takaki; Moriyama, Saya; Matsumura, Takayuki; Isogawa, Masanori; Terahara, Kazutaka; Takano, Tomohiro; Sun, Lin; Nishiyama, Ayae; Omoto, Shinnya; Shinkai, Masaharu; Kurosaki, Tomohiro; Yamashita, Kazuo;
  • imprint: American Association for the Advancement of Science (AAAS), 2023
  • Published in: Science Advances
  • Language: English
  • DOI: 10.1126/sciadv.adf0661
  • ISSN: 2375-2548
  • Keywords: Multidisciplinary
  • Origination:
  • Footnote:
  • Description: <jats:p> Severe acute respiratory syndrome coronavirus 2–neutralizing antibodies primarily target the spike receptor binding domain (RBD). However, B cell antigen receptors (BCRs) on RBD-binding memory B (B <jats:sub>mem</jats:sub> ) cells have variation in the neutralizing activities. Here, by combining single B <jats:sub>mem</jats:sub> cell profiling with antibody functional assessment, we dissected the phenotype of B <jats:sub>mem</jats:sub> cell harboring the potently neutralizing antibodies in coronavirus disease 2019 (COVID-19)–convalescent individuals. The neutralizing subset was marked by an elevated CD62L expression and characterized by distinct epitope preference and usage of convergent V <jats:sub>H</jats:sub> (variable region of immunoglobulin heavy chain) genes, accounting for the neutralizing activities. Concordantly, the correlation was observed between neutralizing antibody titers in blood and CD62L <jats:sup>+</jats:sup> subset, despite the equivalent RBD binding of CD62L <jats:sup>+</jats:sup> and CD62L <jats:sup>−</jats:sup> subset. Furthermore, the kinetics of CD62L <jats:sup>+</jats:sup> subset differed between the patients who recovered from different COVID-19 severities. Our B <jats:sub>mem</jats:sub> cell profiling reveals the unique phenotype of B <jats:sub>mem</jats:sub> cell subset that harbors potently neutralizing BCRs, advancing our understanding of humoral protection. </jats:p>
  • Access State: Open Access