An Extract of Ginkgo Biloba Leaves Attenuates Neurotoxic Changes in Rats with Chronic Fluorosis, as Well as in Sh-Sy5y Cells Exposed to a High Level of Fluoride

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Titel: An Extract of Ginkgo Biloba Leaves Attenuates Neurotoxic Changes in Rats with Chronic Fluorosis, as Well as in Sh-Sy5y Cells Exposed to a High Level of Fluoride

Beteiligte: Xiang, Jie [VerfasserIn]; Ma, Yan Ling [VerfasserIn]; Zhou, Jian [VerfasserIn]; Zeng, Xiao Xiao [VerfasserIn]; Xiao, Xiao [VerfasserIn]; Yu, Yan Long [VerfasserIn]; Dong, Yang-Ting [VerfasserIn]; Ran, Long Yan [VerfasserIn]; Qi, Xiao Lan [VerfasserIn]; Hong, Wei [VerfasserIn]; Gao, Yan Hui [VerfasserIn]; Guan, Zhi-Zhong [VerfasserIn]

Erschienen: [S.l.]: SSRN, [2022]

Umfang: 1 Online-Ressource (28 p)

Sprache: Englisch

DOI: 10.2139/ssrn.4028392

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Beschreibung: Background: Potential protection against the neurotoxic effects of high levels of fluoride on rats and SH-SY5Y cells by an extract of Ginkgo biloba , as well as underlying mechanisms, were examined.Methods: The rats were divided into 4 groups, i.e., control, treatment with the extract (100 mg/kg body weight, gavage once daily), treatment with fluoride (50 ppm F- in drinking water) and combined treatment with both; SH-SY5Y cells exposed to fluoride and fluoride in combination with the extract or 4-ANI, an inhibitor of poly (ADP-ribose) polymerase-1 (PARP-1). Spatial learning and memory in the rats were assessed employing Morris water maze; cytotoxicity of fluoride using CCK-8; activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) by appropriate kits; the content of ROS and frequency of apoptosis by flow cytometry; expressions of phospho-histone H2A.X(Ser139), PARP-1, poly (ADP-ribose) (PAR) and Sirtuin-1 (SIRT1) by Western blotting or immunofluorescence.Results: The learning and memory of the rats with chronic fluorosis declined significantly. In the hippocampus of these rats or in SH-SY5Y cells exposed to fluoride, the contents of ROS and MDA, frequency of apoptosis, and levels of histone H2A.X(Ser139), PARP-1 and PAR were all elevated; the activities of SOD and GSH-Px and level of SIRT1 protein reduced. Simultaneous treatment of the rats exposed to fluoride with the Ginkgo biloba extract attenuated the impairment of learning and memory, as well as all of the neurotoxic effects induced in the brain and in SH-SY5Y cells exposed to fluoride and this protection could be mimicked by exposure of the cells to 4-ANI.Conclusion: The Ginkgo biloba extract attenuated neurotoxic damage to rats with chronic fluorosis and SH-SY5Y cells exposed to fluoride and the underlying mechanism may involve inhibition of PARP-1 activity and promotion of the expression of SIRT1

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