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Medientyp:
Buch
Titel:
Mitochondria
:
practical protocols
Enthält:
Guide to computational methods for predicting mitochondrial localization
/ Su Sun and Bianca H. Habermann
Isolation of functional mitochondria from cultured cells and mouse tissues
/ Jennifer Wettmarshausen and Fabiana Perocchi
Isolation of mitochondria from Saccharomyces cerevisiae
/ Toshiaki Izawa and Ann-Katrin Unger
Isolation of contact sites between inner and outer mitochondrial membranes
/ Max Harner
Isolation of mitochondria-associated membranes (MAM) from mouse brain tissue
/ Bernadette Schreiner and Maria Ankarcrona
Label-free quantitative analysis of mitochondrial proteomes using the multienzyme digestion-filter aided sample preparation (MED-FASP) and "Total Protein Approach"
/ Jacek R. Wiśniewski
Quantitative analysis of glycerophospholipids in mitochondria by mass spectrometry
/ Takashi Tatsuta
Detection of cysteine redox states in mitochondrial proteins in intact mammalian cells
/ Markus Habich and Jan Riemer
Chemical crosslinking in intact mitochondria
/ Rupa Banerjee, Umut Günsel, and Dejana Mokranjac
Reconstitution of mitochondrial membrane proteins into nanodiscs by cell-free expression
/ Ketan Malhotra and Nathan N. Alder
Detection of dual targeting and dual function of mitochondrial proteins in yeast
/ Reut Ben-Menachem and Ophry Pines
Localizing mRNAs encoding mitochondrial proteins in yeast by fluorescence microscopy and subcellular fractionation
/ Dmitry Zabezhinsky, Hannah Sperber, and Jeffrey E. Gerst
Assessing mitochondrial bioenergetics in isolated mitochondria from various mouse tissues using seahorse XF96 analyzer
/ Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, and Holger Prokisch
Application of FRET-based biosensor "ATeam" for visualization of ATP levels in the mitochondrial matrix of living mammalian cells
/ Tomoki Yoshida, Soaad Alfaqaan, Norio Sasaoka, and Hiromi Imamura
Microplate-based bioluminescence assay of mitochondrial calcium uptake
/ María Teresa Alonso, Paloma Navas-Navarro, and Javier García-Sancho
New imaging tools to analyze mitochondrial morphology in Caenorhabditis elegans
/ Saroj G. Regmi and Stéphane G. Rolland
Single molecule tracking and localization of mitochondrial protein complexes in live cells
/ Timo Appelhans and Karin Busch
Analysis of yeast mitochondria by electron microscopy
/ Ann-Katrin Unger, Stefan Geimer, Max Harner, Walter Neupert, and Benedikt Westermann
Analysis of mitochondrial membrane protein complexes by electron cryo-tomography
/ Vicki A.M. Gold, Tobias Brandt, Laetitia Cavellini, Mickael M. Cohen, Raffaele Ieva, and Martin van der Laan
Assays for Mitophagy in Yeast
/ Akinori Eiyama and Koji Okamoto
Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans
/ Konstantinos Palikaras and Nektarios Tavernarakis
Assessing mitochondrial unfolded protein response in mammalian cells
/ Fiona Durand and Nicholas Hoogenraad
Analysis of mitochondrial RNA-processing defects in patient-derived tissues by qRT-PCR and RNAseq
/ Robert Kopajtich, Johannes A. Mayr, and Holger Prokisch
Identification of disease-causing mutations by functional complementation of patient-derived fibroblast cell lines
/ Laura S. Kremer and Holger Prokisch.