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Medientyp:
E-Artikel
Titel:
Optical pH Sensor Covering the Range from pH 0−14 Compatible with Mobile-Device Readout and Based on a Set of Rationally Designed Indicator Dyes
Erschienen:
BAM-Publica - Publikationsserver der Bundesanstalt für Materialforschung und -prüfung (BAM), 2017
Sprache:
Englisch
DOI:
https://doi.org/10.1021/acs.analchem.7b01903
Entstehung:
Anmerkungen:
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Beschreibung:
In this work, a family of pH-responsive fluorescent probes has been designed in a rational manner with the aid of quantum chemistry tools, covering the entire pH range from 0−14. Relying on the boron−dipyrromethene (BODIPY) core, all the probes as well as selected reference dyes display very similar spectroscopic properties with ON−OFF fluorescence switching responses, facilitating optical readout in simple devices used for detection and analysis. Embedding of the probes and reference dyes into hydrogel spots on a plastic strip yielded a test strip that reversibly indicates pH with a considerably small uncertainty of ~0.1 pH units. These strips are not only reusable but, combined with a 3D-printed case that can be attached to a smartphone, the USB port of which drives the integrated LED used for excitation, allows for autonomous operation in on-site or in-the-field applications; the developed Android application software (“app”) further simplifies operation for unskilled users.