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Medientyp:
E-Artikel
Titel:
Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases
Beteiligte:
Meyer, Melanie;
de Angelis, Martin Hrabé;
Wurst, Wolfgang;
Kühn, Ralf
Erschienen:
Proceedings of the National Academy of Sciences, 2010
Erschienen in:Proceedings of the National Academy of Sciences
Sprache:
Englisch
DOI:
10.1073/pnas.1009424107
ISSN:
0027-8424;
1091-6490
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we explored whether gene targeting can be directly performed in zygotes by the use of zinc-finger nucleases. Here we report that gene targeting is achieved in 1.7–4.5% of murine one-cell embryos upon the coinjection of targeting vectors with zinc-finger nucleases, without preselection. These findings enable the manipulation of the mammalian germ line in a single step in zygotes, independent of ES cells.</jats:p>