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Medientyp:
E-Artikel
Titel:
Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato
Beteiligte:
Tai, Thomas H.;
Dahlbeck, Douglas;
Clark, Eszter T.;
Gajiwala, Paresh;
Pasion, Romela;
Whalen, Maureen C.;
Stall, Robert E.;
Staskawicz, Brian J.
Erschienen:
Proceedings of the National Academy of Sciences, 1999
Erschienen in:Proceedings of the National Academy of Sciences
Beschreibung:
<jats:p>
The
<jats:italic>Bs2</jats:italic>
resistance gene of pepper specifically recognizes and confers resistance to strains of
<jats:italic>Xanthomonas campestris</jats:italic>
pv.
<jats:italic>vesicatoria</jats:italic>
that contain the corresponding bacterial avirulence gene,
<jats:italic>avrBs2</jats:italic>
. The involvement of
<jats:italic>avrBs2</jats:italic>
in pathogen fitness and its prevalence in many
<jats:italic>X. campestris</jats:italic>
pathovars suggests that the
<jats:italic>Bs2</jats:italic>
gene may be durable in the field and provide resistance when introduced into other plant species. Employing a positional cloning strategy, the
<jats:italic>Bs2</jats:italic>
locus was isolated and the gene was identified by coexpression with
<jats:italic>avrBs2</jats:italic>
in an
<jats:italic>Agrobacterium</jats:italic>
-mediated transient assay. A single candidate gene, predicted to encode motifs characteristic of the nucleotide binding site–leucine-rich repeat class of resistance genes, was identified. This gene specifically controlled the hypersensitive response when transiently expressed in susceptible pepper and tomato lines and in a nonhost species,
<jats:italic>Nicotiana benthamiana</jats:italic>
, and was designated as
<jats:italic>Bs2</jats:italic>
. Functional expression of
<jats:italic>Bs2</jats:italic>
in stable transgenic tomatoes supports its use as a source of resistance in other Solanaceous plant species.
</jats:p>