A CMV-induced adaptive human Vδ1+ γδ T cell clone recognizes HLA-DR

Medientyp: E-Artikel

Titel: A CMV-induced adaptive human Vδ1+ γδ T cell clone recognizes HLA-DR

Beteiligte: Deseke, Malte; Rampoldi, Francesca; Sandrock, Inga; Borst, Eva; Böning, Heike; Ssebyatika, George Liam; Jürgens, Carina; Plückebaum, Nina; Beck, Maleen; Hassan, Ahmed; Tan, Likai; Demera, Abdi; Janssen, Anika; Steinberger, Peter; Koenecke, Christian; Viejo-Borbolla, Abel; Messerle, Martin; Krey, Thomas; Prinz, Immo

Erschienen: Rockefeller University Press, 2022

Sprache: Englisch

DOI: 10.1084/jem.20212525

ISSN: 0022-1007; 1540-9538

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Beschreibung: The innate and adaptive roles of γδ T cells and their clonal γδ T cell receptors (TCRs) in immune responses are still unclear. Recent studies of γδ TCR repertoire dynamics showed massive expansion of individual Vδ1+ γδ T cell clones during viral infection. To judge whether such expansion is random or actually represents TCR-dependent adaptive immune responses, information about their cognate TCR ligands is required. Here, we used CRISPR/Cas9-mediated screening to identify HLA-DRA, RFXAP, RFX5, and CIITA as required for target cell recognition of a CMV-induced Vγ3Vδ1+ TCR, and further characterization revealed a direct interaction of this Vδ1+ TCR with the MHC II complex HLA-DR. Since MHC II is strongly upregulated by interferon-γ, these results suggest an inflammation-induced MHC-dependent immune response of γδ T cells.

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