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Eckert‐Boulet, Nadine; Nielsen, Peter Stein; Friis, Carsten; dos Santos, Margarida Moreira; Nielsen, Jens; Kielland‐Brandt, Morten C.; Regenberg, Birgitte

Transcriptional profiling of extracellular amino acid sensing in Saccharomyces cerevisiae and the role of Stp1p and Stp2p

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  • Medientyp: E-Artikel
  • Titel: Transcriptional profiling of extracellular amino acid sensing in Saccharomyces cerevisiae and the role of Stp1p and Stp2p
  • Beteiligte: Eckert‐Boulet, Nadine; Nielsen, Peter Stein; Friis, Carsten; dos Santos, Margarida Moreira; Nielsen, Jens; Kielland‐Brandt, Morten C.; Regenberg, Birgitte
  • Erschienen: Wiley, 2004
  • Erschienen in: Yeast
  • Sprache: Englisch
  • DOI: 10.1002/yea.1120
  • ISSN: 0749-503X; 1097-0061
  • Schlagwörter: Genetics ; Applied Microbiology and Biotechnology ; Biochemistry ; Bioengineering ; Biotechnology
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  • Beschreibung: <jats:title>Abstract</jats:title><jats:p><jats:italic>S. cerevisiae</jats:italic> responds to the presence of amino acids in the environment through the membrane‐bound complex SPS, by altering transcription of several genes. Global transcription analysis shows that 46 genes are induced by <jats:sc>L</jats:sc>‐citrulline. Under the given conditions there appears to be only one pathway for induction with <jats:sc>L</jats:sc>‐citrulline, and this pathway is completely dependent on the SPS component, Ssy1p, and either of the transcription factors, Stp1p and Stp2p. Besides the effects on amino acid permease genes, an <jats:italic>ssy1</jats:italic> and an <jats:italic>stp1 stp2</jats:italic> mutant exhibit a number of other transcriptional phenotypes, such as increased expression of genes subject to nitrogen catabolite repression and genes involved in stress response. A group of genes involved in the upper part of the glycolysis, including those encoding hexose transporters Hxt4p, Hxt5p, Hxt6p, Hxt7p, hexokinase Hxk1p, glyceraldehyde 3‐phosphate dehydrogenase Tdh1p and glucokinase (Glk1p), shows increased transcription levels in either or both of the mutants. Also, most of the structural genes involved in trehalose and glycogen synthesis and a few genes in the glyoxylate cycle and the pentose phosphate pathway are derepressed in the <jats:italic>ssy1</jats:italic> and <jats:italic>stp1 stp2</jats:italic> strains. Copyright © 2004 John Wiley &amp; Sons, Ltd.</jats:p>
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