Characterization and Purification of Mouse Mucosal‐Associated Invariant T (MAIT) Cells

Medientyp: E-Artikel
Titel: Characterization and Purification of Mouse Mucosal‐Associated Invariant T (MAIT) Cells
Beteiligte: Chen, Zhenjun; Wang, Huimeng; D'Souza, Criselle; Koay, Hui‐Fern; Meehan, Bronwyn; Zhao, Zhe; Pediongco, Troi; Shi, Mai; Zhu, Tianyuan; Wang, Bingjie; Kjer‐Nielsen, Lars; Eckle, Sidonia B. G.; Rossjohn, Jamie; Fairlie, David P.; Godfrey, Dale I.; Strugnell, Richard A.; McCluskey, James; Corbett, Alexandra J.
Erschienen: Wiley, 2019
Erschienen in: Current Protocols in Immunology
Sprache: Englisch
DOI: 10.1002/cpim.89
ISSN: 1934-3671; 1934-368X
Schlagwörter: Immunology
Entstehung:
Anmerkungen:
Beschreibung: <jats:title>Abstract</jats:title><jats:p>This unit describes the utility of various mouse models of infection and immunization for studying mucosal‐associated invariant T (MAIT) cell immunity: MAIT cells can be isolated from the lungs (or from other tissues/organs) and then identified and characterized by flow cytometry using MR1 tetramers in combination with a range of antibodies. The response kinetics, cytokine profiles, and functional differentiation of lung MAIT cells are studied following infection with the bacterial pathogen<jats:italic>Legionella longbeachae</jats:italic>or<jats:italic>Salmonella enterica</jats:italic>Typhimurium or immunization with synthetic MAIT cell antigen plus Toll‐like receptor agonist. MAIT cells enriched or expanded during the process can be used for further studies. A step‐by‐step protocol is provided for MAIT cell sorting and adoptive transfer. Mice can then be challenged and MAIT cells tracked and further examined. © 2019 by John Wiley & Sons, Inc.</jats:p>

Nur in Feld suchen:

Zuletzt gesuchte Begriffe: