> Merkliste Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp: E-Artikel Titel: The role of temperature, pH and nutrition in process development of the unique oleaginous yeast Metschnikowia pulcherrima Beteiligte: Abeln, Felix; Chuck, Christopher J Erschienen: Wiley, 2020 Erschienen in: Journal of Chemical Technology & Biotechnology Sprache: Englisch DOI: 10.1002/jctb.6301 ISSN: 0268-2575; 1097-4660 Schlagwörter: Inorganic Chemistry ; Organic Chemistry ; Pollution ; Waste Management and Disposal ; Fuel Technology ; Renewable Energy, Sustainability and the Environment ; General Chemical Engineering ; Biotechnology Entstehung: Anmerkungen: Beschreibung: <jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND</jats:title><jats:p>Lipids produced from oleaginous yeasts are a promising alternative to terrestrial oils. Despite promising cellular yields of lipid however, an industrial process remains elusive. One key processing bottleneck is the need to provide nutrient‐rich conditions for cellular growth and then extended nutrient‐depleted conditions for lipid accumulation. Surprisingly, investigations detailing process development, particularly with a focus on kinetics, are rare in this field.</jats:p></jats:sec><jats:sec><jats:title>RESULTS</jats:title><jats:p>In this investigation we report on the unique oleaginous yeast <jats:styled-content style="fixed-case"><jats:italic>Metschnikowia pulcherrima</jats:italic></jats:styled-content>, where lipid accumulation ≥ 29.8% (<jats:italic>w</jats:italic>/<jats:italic>w</jats:italic>) was achieved without apparent nutrient limitation. The process was developed in stirred tank reactors through determining the influence of temperature, pH and nutrition on lipid production. A temperature of up to 25 °C and initial pH 5 could be applied to enhance initial reaction kinetics. Through the increased supply of yeast extract of up to 5% (<jats:italic>w</jats:italic>/<jats:italic>w</jats:italic>) of glucose, a maximum lipid production rate of 0.60 g L<jats:sup>−1</jats:sup> h<jats:sup>−1</jats:sup> (4 h‐average), productivity of 0.29 g L<jats:sup>−1</jats:sup> h<jats:sup>−1</jats:sup>, and yield of 0.17 g g<jats:sup>−1</jats:sup> glucose were achieved – the highest yet recorded with this yeast. Suitable to combat the excessive secretion of polyols of up to 0.11 g g<jats:sup>−1</jats:sup> glucose was an excess nutrient supply as well as a low cultivation temperature of 15 °C and moderate pH 5.</jats:p></jats:sec><jats:sec><jats:title>CONCLUSIONS</jats:title><jats:p>This study demonstrates that the lack of a starvation stage, coupled with effective process development is required for oleaginous yeasts to achieve the yields and productivities required for commercial lipid production. © 2019 Society of Chemical Industry</jats:p></jats:sec>