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Medientyp: E-Artikel Titel: Radial shock waves effectively introduced NF‐kappa B decoy into rat achilles tendon cells in vitro Beteiligte: Sugioka, Kaori; Nakagawa, Koichi; Murata, Ryo; Ochiai, Nobuyasu; Sasho, Takahisa; Arai, Momoko; Tsuruoka, Hiroaki; Ohtori, Seiji; Saisu, Takashi; Gemba, Takefumi; Takahashi, Kazuhisa Erschienen: Wiley, 2010 Erschienen in: Journal of Orthopaedic Research Sprache: Englisch DOI: 10.1002/jor.21081 ISSN: 0736-0266; 1554-527X Schlagwörter: Orthopedics and Sports Medicine Entstehung: Anmerkungen: Beschreibung: <jats:title>Abstract</jats:title><jats:p>The purpose of this study was to test if radial shock waves could enhance the introduction of nuclear factor‐kappa B (NF‐κB) decoy oligodeoxynucleotides, which is reported to markedly inhibit NF‐κB activation and suppress pro‐inflammatory cytokine gene expression, using rat Achilles tendon cells. In the presence of NF‐κB decoy labeled with or without fluorescein isothiocyanate (FITC) in culture media, radial shock waves were applied to the tendon cells in variable conditions and cultivated for 24 h. The transfection rate was assessed by counting FITC‐positive cells, and IL‐1–induced NF‐κB activation in the cells was assessed. Radial shock waves significantly enhanced introduction of NF‐κB decoy‐FITC into the tendon cells. IL‐1–induced NF‐κB activation was significantly inhibited by pretreatment of the cells with NF‐κB decoy combined with radial shock wave exposure. The present study demonstrated the effectiveness of radial shock waves on introduction of NF‐κB decoy into tendon cells. Radial shock wave treatment combined with local NF‐κB decoy administration could be a novel therapeutic strategy for chronic tendinopathy. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1078–1083, 2010</jats:p>