Beschreibung:
<jats:title>Abstract</jats:title><jats:p>Immobilized pH gradient isoelectric focusing (IPG‐IEF) has emerged as a highly promising alternative to strong‐cation exchange fractionation as the first dimension in shot‐gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.</jats:p>