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Dewald, Felix; Suárez, Isabelle; Johnen, Ronja; Grossbach, Jan; Moran-Tovar, Roberto; Steger, Gertrud; Joachim, Alexander; Rubio, Gibran Horemheb; Fries, Mira; Behr, Florian; Kley, Joao; Lingnau, Andreas; Kretschmer, Alina; Gude, Carina; Baeza-Flores, Guadelupe; del Valle, David Laveaga; Roblero-Hernandez, Alberto; Magana-Cerino, Jesus; Hernandez, Adriana Torres; Ruiz-Quinones, Jesus; Schega, Konstantin; Linne, Viktoria; Junker, Lena; Wunsch, Marie; [...]

Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children

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  • Medientyp: E-Artikel
  • Titel: Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children
  • Beteiligte: Dewald, Felix; Suárez, Isabelle; Johnen, Ronja; Grossbach, Jan; Moran-Tovar, Roberto; Steger, Gertrud; Joachim, Alexander; Rubio, Gibran Horemheb; Fries, Mira; Behr, Florian; Kley, Joao; Lingnau, Andreas; Kretschmer, Alina; Gude, Carina; Baeza-Flores, Guadelupe; del Valle, David Laveaga; Roblero-Hernandez, Alberto; Magana-Cerino, Jesus; Hernandez, Adriana Torres; Ruiz-Quinones, Jesus; Schega, Konstantin; Linne, Viktoria; Junker, Lena; Wunsch, Marie; [...]
  • Erschienen: Springer Science and Business Media LLC, 2022
  • Erschienen in: Nature Communications
  • Sprache: Englisch
  • DOI: 10.1038/s41467-022-30664-2
  • ISSN: 2041-1723
  • Schlagwörter: General Physics and Astronomy ; General Biochemistry, Genetics and Molecular Biology ; General Chemistry ; Multidisciplinary
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  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>Systematic SARS-CoV-2 testing is a valuable tool for infection control and surveillance. However, broad application of high sensitive RT-qPCR testing in children is often hampered due to unpleasant sample collection, limited RT-qPCR capacities and high costs. Here, we developed a high-throughput approach (‘Lolli-Method’) for SARS-CoV-2 detection in children, combining non-invasive sample collection with an RT-qPCR-pool testing strategy. SARS-CoV-2 infections were diagnosed with sensitivities of 100% and 93.9% when viral loads were &gt;10<jats:sup>6</jats:sup> copies/ml and &gt;10<jats:sup>3</jats:sup> copies/ml in corresponding Naso-/Oropharyngeal-swabs, respectively. For effective application of the Lolli-Method in schools and daycare facilities, SEIR-modeling indicated a preferred frequency of two tests per week. The developed test strategy was implemented in 3,700 schools and 698 daycare facilities in Germany, screening over 800,000 individuals twice per week. In a period of 3 months, 6,364 pool-RT-qPCRs tested positive (0.64%), ranging from 0.05% to 2.61% per week. Notably, infections correlated with local SARS-CoV-2 incidences and with a school social deprivation index. Moreover, in comparison with the alpha variant, statistical modeling revealed a 36.8% increase for multiple (≥2 children) infections per class following infections with the delta variant. We conclude that the Lolli-Method is a powerful tool for SARS-CoV-2 surveillance and can support infection control in schools and daycare facilities.</jats:p>
  • Zugangsstatus: Freier Zugang