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Aghamiri, Samira; Amirmozafari, Nour; Fallah Mehrabadi, Jalil; Fouladtan, Babak; Samadi Kafil, Hossein

Antibiotic Resistance Pattern and Evaluation of Metallo-Beta Lactamase Genes Includingbla-IMPandbla-VIMTypes inPseudomonas aeruginosaIsolated from Patients in Tehran Hospitals

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  • Medientyp: E-Artikel
  • Titel: Antibiotic Resistance Pattern and Evaluation of Metallo-Beta Lactamase Genes Includingbla-IMPandbla-VIMTypes inPseudomonas aeruginosaIsolated from Patients in Tehran Hospitals
  • Beteiligte: Aghamiri, Samira; Amirmozafari, Nour; Fallah Mehrabadi, Jalil; Fouladtan, Babak; Samadi Kafil, Hossein
  • Erschienen: Hindawi Limited, 2014
  • Erschienen in: ISRN Microbiology
  • Sprache: Englisch
  • DOI: 10.1155/2014/941507
  • ISSN: 2090-7486
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Beta-lactamase producing strains of<jats:italic>Pseudomonas aeruginosa</jats:italic>are important etiological agents of hospital infections. Carbapenems are among the most effective antibiotics used against<jats:italic>Pseudomonas</jats:italic>infections, but they can be rendered infective by group B<jats:italic>β</jats:italic>-lactamase, commonly called metallo-beta lactamase. In this study, the antimicrobial sensitivity patterns of<jats:italic>P. aeruginosa</jats:italic>strains isolated from 9 different hospitals in Tehran, Iran, as well as the prevalence of MBLs genes (<jats:italic>bla</jats:italic>-<jats:sub><jats:italic>VIM</jats:italic></jats:sub>and<jats:italic>bla</jats:italic>-<jats:sub><jats:italic>IMP</jats:italic></jats:sub>) were determined. A total of 212 strains of<jats:italic>P. aeruginosa</jats:italic>recovered from patients in hospitals in Tehran were confirmed by both biochemical methods and PCR. Their antimicrobial sensitivity patterns were determined by Kirby-Bauer disk diffusion method. Following MIC determination, imipenem resistant strains were selected by DDST method which was followed by PCR tests for determination of MBLs genes:<jats:italic>bla</jats:italic>-<jats:sub><jats:italic>IMP</jats:italic></jats:sub>and<jats:italic>bla</jats:italic>-<jats:sub><jats:italic>VIM</jats:italic></jats:sub>. The results indicated that, in the DDST phenotypic method, among the 100 imipenem resistant isolates, 75 strains were MBLs positive. The PCR test indicated that 70 strains (33%) carried<jats:italic>bla</jats:italic>-<jats:sub><jats:italic>VIM</jats:italic></jats:sub>gene and 20 strains (9%) harbored<jats:italic>bla</jats:italic>-<jats:sub><jats:italic>IMP</jats:italic></jats:sub>. The results indicated that the extent of antibiotic resistance among<jats:italic>Pseudomonas aeruginosa</jats:italic>is on the rise. This may be due to production of MBLs enzymes. Therefore, determination of antibiotic sensitivity patterns and MBLs production by these bacteria, can be important in control of clinical<jats:italic>Pseudomonas</jats:italic>infection.</jats:p>