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Aghamiri, Samira; Amirmozafari, Nour; Fallah Mehrabadi, Jalil; Fouladtan, Babak; Hanafi Abdar, Mojtaba

Antibiotic Resistance Patterns and a Survey of Metallo-β-Lactamase Genes Including bla-IMP and bla-VIM Types in Acinetobacter baumannii Isolated from Hospital Patients in Tehran

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  • Medientyp: E-Artikel
  • Titel: Antibiotic Resistance Patterns and a Survey of Metallo-β-Lactamase Genes Including bla-IMP and bla-VIM Types in Acinetobacter baumannii Isolated from Hospital Patients in Tehran
  • Beteiligte: Aghamiri, Samira; Amirmozafari, Nour; Fallah Mehrabadi, Jalil; Fouladtan, Babak; Hanafi Abdar, Mojtaba
  • Erschienen: S. Karger AG, 2016
  • Erschienen in: Chemotherapy
  • Sprache: Englisch
  • DOI: 10.1159/000443825
  • ISSN: 0009-3157; 1421-9794
  • Schlagwörter: Infectious Diseases ; Pharmacology (medical) ; Drug Discovery ; Pharmacology ; Oncology ; General Medicine
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  • Beschreibung: <jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Metallo-β-lactamases (MBLs) producing strains of &lt;i&gt;Acinetobacter baumannii&lt;/i&gt; are serious etiological agents of hospital infections worldwide. Among the β- lactams, carbapenems are the most effective antibiotics used against &lt;i&gt;A. baumannii. &lt;/i&gt;However, resistance to these drugs among clinical strains of &lt;i&gt;A. baumannii&lt;/i&gt; has been increasing in recent years. In this study, the antimicrobial sensitivity patterns of &lt;i&gt;A. baumannii&lt;/i&gt; strains isolated from eleven different hospitals in Tehran, Iran, and the prevalence of MBL genes (&lt;i&gt;bla-&lt;/i&gt;&lt;sub&gt;&lt;i&gt;VIM&lt;/i&gt;&lt;/sub&gt; and &lt;i&gt;bla-&lt;/i&gt;&lt;sub&gt;&lt;i&gt;IMP&lt;/i&gt;&lt;/sub&gt;) were determined. &lt;b&gt;&lt;i&gt;Method:&lt;/i&gt;&lt;/b&gt; During a period of 5 months, 176 isolates of &lt;i&gt;A. baumannii&lt;/i&gt; were collected from different clinical specimens from hospitalized patients in Tehran. All isolates were confirmed by biochemical methods. The isolates were tested for antibiotic sensitivity by the Kirby-Bauer disk diffusion method. Following minimum inhibitory concentration determination, imipenem-resistant isolates were further tested for MBL production by the double disk synergy test (DDST) method. PCR assays were performed for the detection of the MBL genes &lt;i&gt;bla-&lt;/i&gt;&lt;sub&gt;&lt;i&gt;IMP&lt;/i&gt;&lt;/sub&gt; and &lt;i&gt;bla-&lt;/i&gt;&lt;sub&gt;&lt;i&gt;VIM&lt;/i&gt;&lt;/sub&gt;. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; The DDST phenotypic method indicated that among the 169 imipenem-resistant isolates, 165 strains were MBL positive. The PCR assays revealed that 63 of the overall isolates (36%) carried the &lt;i&gt;bla-&lt;/i&gt;&lt;sub&gt;&lt;i&gt;VIM&lt;/i&gt;&lt;/sub&gt; gene and 70 strains (40%) harbored &lt;i&gt;bla-&lt;/i&gt;&lt;sub&gt;&lt;i&gt;IMP&lt;/i&gt;&lt;/sub&gt;. &lt;b&gt;&lt;i&gt;Conclusions:&lt;/i&gt;&lt;/b&gt; It is obvious that nosocomial infections associated with multidrug-resistant &lt;i&gt;Acinetobacter &lt;/i&gt;spp. are on the rise. Therefore, the determination of antibiotic sensitivity patterns and screening for MBL production among &lt;i&gt;A. baumannii&lt;/i&gt; isolates is important for controlling clinical &lt;i&gt;Acinetobacter&lt;/i&gt; infections.</jats:p>