Weihs, Wolfgang;
Ettl, Florian;
Magnet, Ingrid A;
Warenits, Alexandra M;
Herkner, Harald;
Holzer, Michael;
Janata, Andreas;
Högler, Sandra
Abstract 15: Repopulation of CA1 Region in the Hippocampus is Accompanied by Increased Diameter and Reduced Glial Scarring After 20 Weeks of Survival in an 8 Min Ventricular Fibrillation Cardiac Arrest Rat Model
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Medientyp:
E-Artikel
Titel:
Abstract 15: Repopulation of CA1 Region in the Hippocampus is Accompanied by Increased Diameter and Reduced Glial Scarring After 20 Weeks of Survival in an 8 Min Ventricular Fibrillation Cardiac Arrest Rat Model
Beteiligte:
Weihs, Wolfgang;
Ettl, Florian;
Magnet, Ingrid A;
Warenits, Alexandra M;
Herkner, Harald;
Holzer, Michael;
Janata, Andreas;
Högler, Sandra
Beschreibung:
<jats:p>
<jats:bold>Background:</jats:bold>
The CA1 region of hippocampus is specifically vulnerable to global ischemia and is depopulated 14 days after 8min ventricular fibrillation cardiac arrests (VF-CA) and conventional resuscitation in a rat model. We have previously reported on the pyramidal cell repopulation of the CA1 region after long term survival after cardiac arrest in two thirds of animals. (Circulation. 2014;130:A80) The aim of this study was to investigate the distribution of microglia and astrocytes in the CA1 region of rat hippocampus after CA and to measure its diameter.
</jats:p>
<jats:p>
<jats:bold>Methods:</jats:bold>
After 8 min of VF-CA male Sprague-Dawley rats received mechanical chest compressions for 2 min and epinephrine 20 μg/kg. The animals were defibrillated thereafter every 2 min to achieve return of spontaneous circulation. Animals surviving with favorable neurologic recovery were sacrificed 140 days after CA (n=12, 8 with neuronal repopulation (R), 4 without R (nonR)) and compared to 14 day survivors (n=6) and control animals (n=20).
</jats:p>
<jats:p>
<jats:bold>Results:</jats:bold>
Microglia cells/ 75000μm
<jats:sup>2</jats:sup>
were 6±3 in controls, 53±9 in 14d survivors (p<0.001 vs controls), 12±4 in 140d-R and 19±12 in 140d-nonR. Astrocytes/75000μm
<jats:sup>2</jats:sup>
were 9±2 in controls, 15±3 in 14d survivors (p<0.001 vs controls), 21±4 in 140d-R and 43±13 in 140d-nonR animals (p<0.001 vs 140d-R). The hippocampal diameter (μm) was 543±36 in controls, 417±36 in 14d survivors (p<0.001 vs controls), 462±39 in 140d-R and 306±86 in 140d-nonR animals (p<0.001 vs 140d-R).
</jats:p>
<jats:p>
<jats:bold>Conclusions:</jats:bold>
The repopulation of pyramid cells in the CA1 that has taken place in two thirds of animals is accompanied by a significant increase in the diameter of the hippocampus and reduced astrocyte numbers compared to nonR-animals. Numbers of microglial cells are significantly increased after 14d survival removing cell debris after neuronal damage. This lifelong potential of the hippocampus for repair due to adult neurogenesis may open an additional late phase therapeutic window for CA recovery.
</jats:p>