Mathioudakis, George;
Storb, Rainer;
McSweeney, Peter A.;
Torok-Storb, Beverly;
Lansdorp, Peter M.;
Brümmendorf, Tim H.;
Gass, M. John;
Bryant, Eileen M.;
Storek, Jan;
Flowers, Mary E. D.;
Gooley, Ted;
Nash, Richard A.
Polyclonal hematopoiesis with variable telomere shortening in human long-term allogeneic marrow graft recipients
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Polyclonal hematopoiesis with variable telomere shortening in human long-term allogeneic marrow graft recipients
Beteiligte:
Mathioudakis, George;
Storb, Rainer;
McSweeney, Peter A.;
Torok-Storb, Beverly;
Lansdorp, Peter M.;
Brümmendorf, Tim H.;
Gass, M. John;
Bryant, Eileen M.;
Storek, Jan;
Flowers, Mary E. D.;
Gooley, Ted;
Nash, Richard A.
Beschreibung:
<jats:p>Donor-derived hematopoiesis was assessed in 17 patients who received allogeneic marrow grafts from HLA-matched siblings between 1971 and 1980. Complete blood counts were normal or near normal in all patients except one. Chimerism analyses, using either dual-color XY-chromosome fluorescence in situ hybridization (FISH) or analysis of variable number tandem repeat loci, indicated that 15 out of 16 patients had greater than 97% donor-derived hematopoiesis, whereas 1 patient had indeterminate chimerism. All 12 recipients of grafts from female donors exhibited polyclonal hematopoiesis by X-linked clonal analysis with the use of molecular probes. Of the 17 recipients, 9 exhibited a less than 1.0-kilobase shortening of granulocyte telomere length compared with their respective donors, according to terminal restriction fragment analysis or flow-FISH with a fluorescein-labeled peptide nucleic acid probe. These data suggest that under standard transplantation conditions, the stem cell proliferative potential is not compromised during hematopoietic reconstitution.</jats:p>