Beschreibung:
<jats:p>Crystals of the 60 kDa dimeric <jats:italic>Hin</jats:italic>cII restriction enzyme bound to a 12 base-pair dyad-symmetric duplex DNA carrying the specific 5′-GTCGAC recognition site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as precipitating agent. The rod-shaped crystals belong to space group <jats:italic>I</jats:italic>222 (or <jats:italic>I</jats:italic>2<jats:sub>1</jats:sub>2<jats:sub>1</jats:sub>2<jats:sub>1</jats:sub>), with unit-cell dimensions <jats:italic>a</jats:italic> = 66.9, <jats:italic>b</jats:italic> = 176.7, <jats:italic>c</jats:italic> = 256.0 Å. There are most likely to be two dimeric complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.5 Å at 100 K, with an <jats:italic>R</jats:italic>
<jats:sub>merge</jats:sub> of 4.8%.</jats:p>