Perez-Berna, Ana J.;
Benseny-Cases, Nuria;
Rodríguez, María José;
Valcarcel, Ricardo;
Carrascosa, José L.;
Gastaminza, Pablo;
Pereiro, Eva
Monitoring reversion of hepatitis C virus-induced cellular alterations by direct-acting antivirals using cryo soft X-ray tomography and infrared microscopy
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Monitoring reversion of hepatitis C virus-induced cellular alterations by direct-acting antivirals using cryo soft X-ray tomography and infrared microscopy
Beteiligte:
Perez-Berna, Ana J.;
Benseny-Cases, Nuria;
Rodríguez, María José;
Valcarcel, Ricardo;
Carrascosa, José L.;
Gastaminza, Pablo;
Pereiro, Eva
Erschienen:
International Union of Crystallography (IUCr), 2021
Erschienen in:Acta Crystallographica Section D Structural Biology
Beschreibung:
<jats:p>Hepatitis C virus (HCV) is an enveloped RNA virus. One of the hallmarks of HCV infection is a rearrangement of the host cell membranes, known as the `membranous web'. Full-field cryo soft X-ray tomography (cryo-SXT) in the water-window energy range (284–543 eV) was performed on the MISTRAL beamline to investigate, in whole unstained cells, the morphology of the membranous rearrangements induced in HCV replicon-harbouring cells in conditions close to the living physiological state. All morphological alterations could be reverted by a combination of sofosbuvir/daclatasvir, which are clinically approved antivirals (direct-acting antivirals; DAAs) for HCV infection. Correlatively combining cryo-SXT and 2D synchrotron-based infrared microscopy provides critical information on the chemical nature of specific infection-related structures, which allows specific patterns of the infection process or the DAA-mediated healing process to be distinguished.</jats:p>