> Detailanzeige

Breidenbach, Björn; Blaser, Martin B.; Klose, Melanie; Conrad, Ralf

Crop rotation of flooded rice with upland maize impacts the resident and active methanogenic microbial community

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Crop rotation of flooded rice with upland maize impacts the resident and active methanogenic microbial community
  • Beteiligte: Breidenbach, Björn; Blaser, Martin B.; Klose, Melanie; Conrad, Ralf
  • Erschienen: Wiley, 2016
  • Erschienen in: Environmental Microbiology
  • Sprache: Englisch
  • DOI: 10.1111/1462-2920.13041
  • ISSN: 1462-2912; 1462-2920
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Summary</jats:title><jats:p>Crop rotation of flooded rice with upland crops is a common management scheme allowing the reduction of water consumption along with the reduction of methane emission. The introduction of an upland crop into the paddy rice ecosystem leads to dramatic changes in field conditions (oxygen availability, redox conditions). However, the impact of this practice on the archaeal and bacterial communities has scarcely been studied. Here, we provide a comprehensive study focusing on the crop rotation between flooded rice in the wet season and upland maize (<jats:styled-content style="fixed-case">RM</jats:styled-content>) in the dry season in comparison with flooded rice (<jats:styled-content style="fixed-case">RR</jats:styled-content>) in both seasons. The composition of the resident and active microbial communities was assessed by 454 pyrosequencing targeting the archaeal and bacterial 16<jats:styled-content style="fixed-case">S rRNA</jats:styled-content> gene and 16<jats:styled-content style="fixed-case">S rRNA</jats:styled-content>. The archaeal community composition changed dramatically in the rotational fields indicated by a decrease of anaerobic methanogenic lineages and an increase of aerobic <jats:styled-content style="fixed-case"><jats:italic>T</jats:italic></jats:styled-content><jats:italic>haumarchaeota</jats:italic>. Members of <jats:styled-content style="fixed-case"><jats:italic>M</jats:italic></jats:styled-content><jats:italic>ethanomicrobiales</jats:italic>, <jats:styled-content style="fixed-case"><jats:italic>M</jats:italic></jats:styled-content><jats:italic>ethanosarcinaceae</jats:italic>, <jats:styled-content style="fixed-case"><jats:italic>M</jats:italic></jats:styled-content><jats:italic>ethanosaetaceae</jats:italic> and <jats:styled-content style="fixed-case"><jats:italic>M</jats:italic></jats:styled-content><jats:italic>ethanocellaceae</jats:italic> were equally suppressed in the rotational fields indicating influence on both acetoclastic and hydrogenotrophic methanogens. On the contrary, members of soil crenarchaeotic group, mainly <jats:styled-content style="fixed-case"><jats:italic>C</jats:italic></jats:styled-content><jats:italic>andidatus</jats:italic> <jats:styled-content style="fixed-case">N</jats:styled-content>itrososphaera, were higher in the rotational fields, possibly indicating increasing importance of ammonia oxidation during drainage. In contrast, minor effects on the bacterial community were observed. <jats:styled-content style="fixed-case"><jats:italic>A</jats:italic></jats:styled-content><jats:italic>cidobacteria</jats:italic> and <jats:styled-content style="fixed-case"><jats:italic>A</jats:italic></jats:styled-content><jats:italic>naeromyxobacter</jats:italic> spp. were enriched in the rotational fields, whereas members of anaerobic <jats:styled-content style="fixed-case"><jats:italic>C</jats:italic></jats:styled-content><jats:italic>hloroflexi</jats:italic> and sulfate‐reducing members of <jats:styled-content style="fixed-case"><jats:italic>D</jats:italic></jats:styled-content><jats:italic>eltaproteobacteria</jats:italic> were found in higher abundance in the rice fields. Combining quantitative polymerase chain reaction and pyrosequencing data revealed increased ribosomal numbers per cell for methanogenic species during crop rotation. This stress response, however, did not allow the methanogenic community to recover in the rotational fields during re‐flooding and rice cultivation. In summary, the analyses showed that crop rotation with upland maize led to dramatic changes in the archaeal community composition whereas the bacterial community was only little affected.</jats:p>