Thomas, William J.;
Thireault, Caitlin A.;
Kimbrel, Jeffrey A.;
Chang, Jeff H.
Recombineering and stable integration of the Pseudomonas syringae pv. syringae 61 hrp/hrc cluster into the genome of the soil bacterium Pseudomonas fluorescens Pf0‐1
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Medientyp:
E-Artikel
Titel:
Recombineering and stable integration of the Pseudomonas syringae pv. syringae 61 hrp/hrc cluster into the genome of the soil bacterium Pseudomonas fluorescens Pf0‐1
Beteiligte:
Thomas, William J.;
Thireault, Caitlin A.;
Kimbrel, Jeffrey A.;
Chang, Jeff H.
Erschienen:
Wiley, 2009
Erschienen in:The Plant Journal
Sprache:
Englisch
DOI:
10.1111/j.1365-313x.2009.03998.x
ISSN:
0960-7412;
1365-313X
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Summary</jats:title><jats:p>Many Gram‐negative bacteria use a type III secretion system (T3SS) to establish associations with their hosts. The T3SS is a conduit for direct injection of type‐III effector proteins into host cells, where they manipulate the host for the benefit of the infecting bacterium. For plant‐associated pathogens, the variations in number and amino acid sequences of type‐III effectors, as well as their functional redundancy, make studying type‐III effectors challenging. To mitigate this challenge, we developed a stable delivery system for individual or defined sets of type‐III effectors into plant cells. We used recombineering and Tn5‐mediated transposition to clone and stably integrate, respectively, the complete <jats:italic>hrp/hrc</jats:italic> region from <jats:italic>Pseudomonas syringae</jats:italic> pv. <jats:italic>syringae</jats:italic> 61 into the genome of the soil bacterium <jats:italic>Pseudomonas fluorescens</jats:italic> Pf0‐1. We describe our development of Effector‐to‐Host Analyzer (EtHAn), and demonstrate its utility for studying effectors for their <jats:italic>in planta</jats:italic> functions.</jats:p>