Beschreibung:
<jats:title>Abstract</jats:title><jats:p>The purpose of the present study was to evaluate the short‐term effects of aflatoxin B<jats:sub>1</jats:sub> (AFB<jats:sub>1</jats:sub>) and deoxynivalenol (DON) exposure on the expression of the genes encoding the glutathione redox system glutathione peroxidase 4a (<jats:italic>gpx4a</jats:italic>), glutathione peroxidase 4b (<jats:italic>gpx4b</jats:italic>), glutathione synthetase (<jats:italic>gss</jats:italic>) and glutathione reductase (<jats:italic>gsr</jats:italic>) and the oxidative stress response‐related transcription factors Kelch‐like ECH‐associated protein 1 (<jats:italic>keap1</jats:italic>) and nuclear factor‐erythroid 2 p45‐related factor 2 (<jats:italic>nrf2</jats:italic>) in liver, kidney and spleen of common carp. During the 24‐hr long experiment, three different doses (5 µg AFB<jats:sub>1</jats:sub> and 110 µg DON; 7.5 µg AFB<jats:sub>1</jats:sub> and 165 µg DON or 10 µg AFB<jats:sub>1</jats:sub> and 220 µg DON/kg bw) were used. The results indicated that the co‐exposure of AFB<jats:sub>1</jats:sub> and DON initiated free radical formation in liver, kidney and spleen, which was suggested by the increase in Nrf2 dependent genes, namely <jats:italic>gpx4a</jats:italic>, <jats:italic>gpx4b</jats:italic>, <jats:italic>gss</jats:italic> and <jats:italic>gsr</jats:italic>. Expression of <jats:italic>keap1</jats:italic> gene showed upregulation after 8 hr of mycotoxin exposure, and also upregulation of <jats:italic>nrf2</jats:italic> gene was found in kidney after 8 hr of exposure, while in the liver, only slight differences were observed. The changes in the expression of the analysed genes suggest that level of reactive oxygen species reached a critical level where other signalling pathway was activated as described by the hierarchical model of oxidative stress.</jats:p>