Tracking cancer drugs in living cells by thermal profiling of the proteome

Medientyp: E-Artikel
Titel: Tracking cancer drugs in living cells by thermal profiling of the proteome
Beteiligte: Savitski, Mikhail M.; Reinhard, Friedrich B. M.; Franken, Holger; Werner, Thilo; Savitski, Maria Fälth; Eberhard, Dirk; Molina, Daniel Martinez; Jafari, Rozbeh; Dovega, Rebecca Bakszt; Klaeger, Susan; Kuster, Bernhard; Nordlund, Pär; Bantscheff, Marcus; Drewes, Gerard
Erschienen: American Association for the Advancement of Science (AAAS), 2014
Erschienen in: Science
Sprache: Englisch
DOI: 10.1126/science.1255784
ISSN: 0036-8075; 1095-9203
Entstehung:
Anmerkungen:
Beschreibung: <jats:title>Mapping human drug targets in the cell</jats:title> <jats:p> To understand both the beneficial and the side effects of a drug, one would need to know its full binding profile to all cellular proteins. Savitski <jats:italic>et al.</jats:italic> take significant steps toward meeting this daunting challenge. They monitored the unfolding or “melting” of over 7000 human proteins and measured how small-molecule binding changes individual melting profiles. As a proof of principle, over 50 targets were identified for an inhibitor known to bind a broad spectrum of kinases. Two cancer drugs, vemurafib and Alectinib, are known to have a side effect of photosensitivity. The thermal profiling approach identified drug-protein interactions responsible for these side effects. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" related-article-type="in-this-issue" xlink:href="10.1126/science.1255784">10.1126/science.1255784</jats:related-article> </jats:p>

Nur in Feld suchen:

Zuletzt gesuchte Begriffe: