Beschreibung:
<jats:title>ABSTRACT</jats:title><jats:p>The effect that multiple percutaneous exposures to<jats:named-content content-type="genus-species">Schistosoma</jats:named-content>larvae has on the development of early CD4<jats:sup>+</jats:sup>lymphocyte reactivity is unclear, yet it is important in the context of humans living in areas where schistosomiasis is endemic. In a murine model of multiple infections, we show that exposure of mice to repeated doses (4×) of<jats:named-content content-type="genus-species">Schistosoma mansoni</jats:named-content>cercariae, compared to a single dose (1×), results in CD4<jats:sup>+</jats:sup>T cell hyporesponsiveness within the skin-draining lymph nodes (sdLN), manifested as reduced CD4<jats:sup>+</jats:sup>cell proliferation and cytokine production. FoxP3<jats:sup>+</jats:sup>CD4<jats:sup>+</jats:sup>regulatory T cells were present in similar numbers in the sdLN of 4× and 1× mice and thus are unlikely to have a role in effecting hyporesponsiveness. Moreover, anergy of the CD4<jats:sup>+</jats:sup>cell population from 4× mice was slight, as proliferation was only partly circumvented through the<jats:italic>in vitro</jats:italic>addition of exogenous interleukin-2 (IL-2), and the<jats:italic>in vivo</jats:italic>blockade of the regulatory molecule PD1 had a minimal effect on restoring responsiveness. In contrast, IL-10 was observed to be critical in mediating hyporesponsiveness, as CD4<jats:sup>+</jats:sup>cells from the sdLN of 4× mice deficient for IL-10 were readily able to proliferate, unlike those from 4× wild-type cohorts. CD4<jats:sup>+</jats:sup>cells from the sdLN of 4× mice exhibited higher levels of apoptosis and cell death, but in the absence of IL-10, there was significantly less cell death. Combined, our data show that IL-10 is a key factor in the development of CD4<jats:sup>+</jats:sup>T cell hyporesponsiveness after repeated parasite exposure involving CD4<jats:sup>+</jats:sup>cell apoptosis.</jats:p>