Beschreibung:
<jats:title>ABSTRACT</jats:title>
<jats:p>
Two copies of IS
<jats:italic>1675</jats:italic>
, a novel lactococcal insertion element from the IS
<jats:italic>4</jats:italic>
family, are present on a 70-kb plasmid, where they frame the lantibiotic lacticin 481 operon. The whole structure could be a composite transposon designated Tn
<jats:italic>5721</jats:italic>
. This study shows that the lacticin 481 operon does not include any regulatory gene and provides a new example of a transposon-associated bacteriocin determinant. We identified five other IS
<jats:italic>1675</jats:italic>
copies not associated with the lacticin 481 operon. The conservation of IS
<jats:italic>1675</jats:italic>
flanking sequences suggested a 24-bp target site.
</jats:p>