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Ploquin, Mickaël J.-Y.; Diop, Ousmane M.; Sol-Foulon, Nathalie; Mortara, Lorenzo; Faye, Abdourahmane; Soares, Marcelo A.; Nerrienet, Eric; Le Grand, Roger; Van Kooyk, Yvette; Amara, Ali; Schwartz, Olivier; Barré-Sinoussi, Françoise; Müller-Trutwin, Michaela C.

DC-SIGN from African Green Monkeys Is Expressed in Lymph Nodes and Mediates Infection intransof Simian Immunodeficiency Virus SIVagm

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  • Medientyp: E-Artikel
  • Titel: DC-SIGN from African Green Monkeys Is Expressed in Lymph Nodes and Mediates Infection intransof Simian Immunodeficiency Virus SIVagm
  • Beteiligte: Ploquin, Mickaël J.-Y.; Diop, Ousmane M.; Sol-Foulon, Nathalie; Mortara, Lorenzo; Faye, Abdourahmane; Soares, Marcelo A.; Nerrienet, Eric; Le Grand, Roger; Van Kooyk, Yvette; Amara, Ali; Schwartz, Olivier; Barré-Sinoussi, Françoise; Müller-Trutwin, Michaela C.
  • Erschienen: American Society for Microbiology, 2004
  • Erschienen in: Journal of Virology
  • Sprache: Englisch
  • DOI: 10.1128/jvi.78.2.798-810.2004
  • ISSN: 0022-538X; 1098-5514
  • Schlagwörter: Virology ; Insect Science ; Immunology ; Microbiology
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>ABSTRACT</jats:title><jats:p>African green monkeys (AGMs) infected by simian immunodeficiency virus (SIV) SIVagm are resistant to AIDS. SIVagm-infected AGMs exhibit levels of viremia similar to those described during pathogenic human immunodeficiency virus type 1 (HIV-1) and SIVmac infections in humans and macaques, respectively, but contain lower viral loads in their lymph nodes. We addressed the potential role of dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN; CD209) in viral dissemination. In previous studies, it has been shown that human DC-SIGN and macaque DC-SIGN allow transmission of HIV and SIVmac to T cells. Here, we looked at the ability of DC-SIGN derived from AGM lymph nodes to interact with SIVagm. We show that DC-SIGN-expressing cells are present mainly in the medulla and often within the cortex and/or paracortex of AGM lymph nodes. We describe the isolation and characterization of at least three isoforms of<jats:italic>dc-sign</jats:italic>mRNA in lymph nodes of AGMs. The predicted amino acid sequence from the predominant mRNA isoform, DC-SIGNagm1, is 92 and 99% identical to the corresponding human and rhesus macaque DC-SIGN amino acid sequences, respectively. DC-SIGNagm1 is characterized by the lack of the fourth motif in the repeat domain. This deletion was also detected in the<jats:italic>dc-sign</jats:italic>gene derived from thirteen animals belonging to five other African monkey species and from four macaques (<jats:italic>Macaca fascicularis</jats:italic>and<jats:italic>M. mulatta</jats:italic>). Despite three- to seven-amino-acid modifications compared to DC-SIGNmac, DC-SIGNagm1 allows transmission of SIVagm to T cells. Furthermore, AGM monocyte-derived dendritic cells (MDDC) expressed at least 100,000 DC-SIGN molecules and were able to transmit SIVagm to T cells. At a low multiplicity of infection (10<jats:sup>−5</jats:sup>50% tissue culture infective doses/cell), viral transmission by AGM MDDC was mainly DC-SIGN dependent. The present study reveals that DC-SIGN from a natural host species of SIV has the ability to act as an efficient attachment and transmission factor for SIVagm and suggests the absence of a direct link between this ability and viral load levels in lymph nodes.</jats:p>
  • Zugangsstatus: Freier Zugang