Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
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Medientyp:
E-Artikel
Titel:
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
Erschienen:
Public Library of Science (PLoS), 2021
Erschienen in:PLOS ONE
Sprache:
Englisch
DOI:
10.1371/journal.pone.0259673
ISSN:
1932-6203
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p><jats:italic>Serratia marcescens</jats:italic>is a species that belongs to the family of<jats:italic>Yersiniaceae</jats:italic>. This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3<jats:sup>R</jats:sup>) of the species<jats:italic>Serratia marcescens</jats:italic>. The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of<jats:italic>Serratia</jats:italic>associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3<jats:sup>R</jats:sup>belongs to the species<jats:italic>Serratia marcescens</jats:italic>.<jats:italic>In silico</jats:italic>analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3<jats:sup>R</jats:sup>showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain<jats:italic>S</jats:italic>.<jats:italic>marcescens</jats:italic>LVF3<jats:sup>R</jats:sup>is a potential PGPR strain. It harbors genes coding for indole acetic acid<jats:bold>(</jats:bold>IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation.</jats:p>