Kraus, Marc S.;
Jesty, Sophy A.;
Gelzer, Anna R.;
Ducharme, Norm G.;
Mohammed, Hussni O.;
Mitchell, Lisa M.;
Soderholm, Leo V.;
Divers, Thomas J.
Measurement of plasma cardiac troponin I concentration by use of a point-of-care analyzer in clinically normal horses and horses with experimentally induced cardiac disease
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Medientyp:
E-Artikel
Titel:
Measurement of plasma cardiac troponin I concentration by use of a point-of-care analyzer in clinically normal horses and horses with experimentally induced cardiac disease
Beteiligte:
Kraus, Marc S.;
Jesty, Sophy A.;
Gelzer, Anna R.;
Ducharme, Norm G.;
Mohammed, Hussni O.;
Mitchell, Lisa M.;
Soderholm, Leo V.;
Divers, Thomas J.
Erschienen:
American Veterinary Medical Association (AVMA), 2010
Erschienen in:American Journal of Veterinary Research
Beschreibung:
<jats:title>Abstract</jats:title>
<jats:p><jats:bold>Objective</jats:bold>—To compare cardiac troponin I (cTnI) concentrations determined by use of a point-of-care analyzer with values determined by use of a bench-top immunoassay in plasma samples obtained from clinically normal horses with and without experimentally induced cardiac disease, and to establish a reference range for plasma equine cTnI concentration determined by use of the point-of-care analyzer.</jats:p>
<jats:p><jats:bold>Animals</jats:bold>—83 clinically normal horses, 6 of which were administered monensin to induce cardiac disease.</jats:p>
<jats:p><jats:bold>Procedures</jats:bold>—A blood sample was collected from each of the 83 clinically normal horses to provide plasma for analysis by use of the point-of-care analyzer; some of the same samples were also analyzed by use of the immunoassay. All 83 samples were used to establish an analyzer-specific reference range for plasma cTnI concentration in clinically normal horses. In 6 horses, blood samples were also collected at various time points after administration of a single dose of monensin (1.0 to 1.5 mg/kg) via nasogastric intubation; plasma cTnI concentration in those samples was assessed by use of both methods.</jats:p>
<jats:p><jats:bold>Results</jats:bold>—The analyzer-specific reference range for plasma cTnI concentration in clinically normal horses was 0.0 to 0.06 ng/mL. Following monensin treatment in 5 horses, increases in plasma cTnI concentration determined by use of the 2 methods were highly correlated (Pearson correlation, 0.83). Peak analyzer-determined plasma cTnI concentrations in monensin-treated horses ranged from 0.08 to 3.68 ng/mL.</jats:p>
<jats:p><jats:bold>Conclusions and Clinical Relevance</jats:bold>—In horses with and without experimentally induced cardiac disease, the point-of-care analyzer and bench-top immunoassay provided similar values of plasma cTnI concentration.</jats:p>