Beschreibung:
<jats:p>Hippuric acid, pronounced as n-benzoyl glycine, is utilized in the study of nutrition, small bioactive molecules, amino acid derivatives, peptide synthesis, chemical synthesis, cell biology, and chemical biology. It can be found in urine and is produced when glycine and benzoic acid combine. Treatment of urinary tract infections (UTIs) is challenging due to the development of resistant bacterial strains brought on by repeated use of antibiotics and chemotherapy. Methenamine hippurate as a pharmaceutical dosage form hasn’t been the subject of any published research, hence a UV-visible spectrophotometric analytical method development has to be created. Hippuric acid (10 g/mL) solution was made, and when it was scanned at 200–400 nm with distilled water as the green solvent, it was discovered that the maximum absorbance was at 228 nm. The linearity concentration range was found with an R2 = 0.998 correlation coeffi cient. The recovery experiment, which was conducted at three levels of 80, 100, and 120%, was used to evaluate the procedure’s accuracy. The recovery rate was discovered to be between 90 and 120%. The % RSD < 2 it proves that the method is Precise. The results confi rmed the approach’s high capacity to detect Hippuric acid and were consistent with industry standards. LoD and LoQ were discovered to be 0.169 and 0.048, respectively. This approach results in hipuuric acid in formulations</jats:p>