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Medientyp:
E-Artikel
Titel:
Intercellular and Intercultural Heterogeneity in Secondary Metabolite Accumulation in Cultures of Catharanthus roseus following Cell Line Selection
Beteiligte:
HALL, ROBERT D.;
YEOMAN, MICHAEL M.
Erschienen:
Clarendon Press, 1987
Erschienen in:Journal of Experimental Botany
Sprache:
Englisch
ISSN:
0022-0957;
1460-2431
Entstehung:
Anmerkungen:
Beschreibung:
<p>Anthocyanin accumulation in a stock culture of Catharanthus roseus was consistently found, using microscopic and microdensitometric techniques, to involve only c. 10% of the cell population. However, an analysis of 26 cell lines isolated from this culture has indicated that all of the cells within the culture were, theoretically, capable of anthocyanin synthesis. Nevertheless, these lines did display substantially different capacities for anthocyanin accumulation. Detailed studies on individual cells from these cultures have revealed that the variation in accumulation was primarily due to differing proportions of pigmented (i.e. productive) cells rather than differing mean intracellular anthocyanin concentrations within these cells. Both the proportion of productive cells and the overall culture yield of the cell lines varied by > 30-fold whereas the mean intracellular anthocyanin concentration varied by < 2-fold. The relevance of these results to the possible control mechanisms involved in secondary metabolite production in this and other culture systems is discussed.</p>