Brown, Darron R.;
Bryan, Janine T.;
Schroeder, Jill M.;
Robinson, Tiffany S.;
Fife, Kenneth H.;
Wheeler, Cosette M.;
Barr, Eliav;
Smith, Paula R.;
Chiacchierini, Lisa;
DiCello, Anthony;
Jansen, Kathrin U.
Neutralization of Human Papillomavirus Type 11 (HPV-11) by Serum from Women Vaccinated with Yeast-Derived HPV-11 LI Virus-like Particles: Correlation with Competitive Radioimmunoassay Titer
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Neutralization of Human Papillomavirus Type 11 (HPV-11) by Serum from Women Vaccinated with Yeast-Derived HPV-11 LI Virus-like Particles: Correlation with Competitive Radioimmunoassay Titer
Beteiligte:
Brown, Darron R.;
Bryan, Janine T.;
Schroeder, Jill M.;
Robinson, Tiffany S.;
Fife, Kenneth H.;
Wheeler, Cosette M.;
Barr, Eliav;
Smith, Paula R.;
Chiacchierini, Lisa;
DiCello, Anthony;
Jansen, Kathrin U.
Beschreibung:
<p>Neutralization of human papillomavirus type 11 (HPV-11) has been demonstrated using serum and cervical secretions from primates vaccinated with virus-like particles (VLPs). Theoretically, neutralizing antibodies could protect women from HPV infection. The immunogenicity of a yeast-derived HPV-11 LI VLP vaccine was tested in women. Serum specimens were evaluated for HPV-11 titer by competitive radioimmunoassay (cRIA) and for neutralization by use of the athymic mouse xenograft system. Analysis of serum from 104 subjects showed a dose response in HPV-11 cRIA titers and neutralization. Overall, 68 (82.9%) of 82 postimmunization serum specimens from VLP recipients were 100% neutralizing when used in the assay at a 1:50 dilution. Of 69 serum specimens, 63 (91.3% with cRIA titers >;200 milliMerck units per milliliter were neutralizing. Immunization with HPV VLPs elicits a vigorous serum immune response in a high percentage of women. The HPV-11 cRIA titer appears to be a surrogate marker for neutralization.</p>