De Lacy Costello, B. P. J.;
Evans, P.;
Ewen, R. J.;
Gunson, H. E.;
Jones, P. R. H.;
Ratcliffe, N. M.;
Spencer‐Phillips, P. T. N.
Gas chromatography–mass spectrometry analyses of volatile organic compounds from potato tubers inoculated with Phytophthora infestans or Fusarium coeruleum
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Media type:
E-Article
Title:
Gas chromatography–mass spectrometry analyses of volatile organic compounds from potato tubers inoculated with Phytophthora infestans or Fusarium coeruleum
Contributor:
De Lacy Costello, B. P. J.;
Evans, P.;
Ewen, R. J.;
Gunson, H. E.;
Jones, P. R. H.;
Ratcliffe, N. M.;
Spencer‐Phillips, P. T. N.
imprint:
Wiley, 2001
Published in:Plant Pathology
Language:
English
DOI:
10.1046/j.1365-3059.2001.00594.x
ISSN:
0032-0862;
1365-3059
Origination:
Footnote:
Description:
<jats:p>Volatile organic compounds (VOCs) collected from potato tubers inoculated with <jats:italic>Phytophthora infestans</jats:italic> (late blight), <jats:italic>Fusarium coeruleum</jats:italic> (dry rot) or sterilized distilled water (as a control) were analysed using gas chromatography–mass spectrometry (GC–MS) and gas chromatography–flame ionization detection (GC–FID). A total of 52 volatiles were identified by GC–MS in the headspaces above <jats:italic>P. infestans‐</jats:italic> and <jats:italic>F. coeruleum‐</jats:italic>inoculated tubers after incubation for 42 days in the dark at 10°C. Of these VOCs, the six most abundant were common to both pathogens. These were benzothiazole (highest abundance), 2‐ethyl‐1‐hexanol (second highest abundance), and at approximately equal third abundance, hexanal, 2‐methylpropanoic acid‐2,2‐dimethyl‐1‐(2‐hydroxy‐1‐methylethyl)‐propyl ester, 2‐methylpropanoic acid‐3‐hydroxy‐2,4,4‐trimethyl‐pentyl ester and phenol. In addition, styrene also occurred at approximately equal third abundance in the headspace of <jats:italic>F. coeruleum‐</jats:italic>inoculated tubers, but at lower abundance in the headspace of <jats:italic>P. infestans‐</jats:italic>inoculated tubers. Some VOCs were specific to each pathogen. Butanal, 3‐methylbutanal, undecane and verbenone were found at low levels only in the headspace of tubers inoculated with <jats:italic>P. infestans</jats:italic>, while 2‐pentylfuran and copaene were found only in the headspace of tubers inoculated with <jats:italic>F. coeruleum</jats:italic>. Additionally GC–FID analysis identified ethanol and 2‐propanol in the liquid exudate from both <jats:italic>P. infestans</jats:italic>‐ and <jats:italic>F. coeruleum</jats:italic>‐inoculated tubers after incubation for 35 days, and in the headspace after incubation for 42 days. These data provide key information for developing a sensor‐based early warning system for the detection of postharvest diseases in stored potato tubers.</jats:p>