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Laizé, Vincent; Rousselet, Germain; Verbavatz, Jean-Marc; Berthonaud, Véronique; Gobin, Renée; Roudier, Nathalie; Abrami, Laurence; Ripoche, Pierre; Tacnet, Frédérique

Functional expression of the human CHIP28 water channel in a yeast secretory mutant

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  • Media type: E-Article
  • Title: Functional expression of the human CHIP28 water channel in a yeast secretory mutant
  • Contributor: Laizé, Vincent; Rousselet, Germain; Verbavatz, Jean-Marc; Berthonaud, Véronique; Gobin, Renée; Roudier, Nathalie; Abrami, Laurence; Ripoche, Pierre; Tacnet, Frédérique
  • imprint: Wiley, 1995
  • Published in: FEBS Letters
  • Language: English
  • DOI: 10.1016/0014-5793(95)01060-r
  • ISSN: 0014-5793; 1873-3468
  • Keywords: Cell Biology ; Genetics ; Molecular Biology ; Biochemistry ; Structural Biology ; Biophysics
  • Origination:
  • Footnote:
  • Description: <jats:p>The temperature‐sensitive <jats:italic>Saccharomyces cerevisiae</jats:italic> mutant strain NY17, deficient in the secretory pathway (<jats:italic>sec6‐4</jats:italic> mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat‐shock, the protein is present in partially purified post‐golgi secretory vesicles. Immunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.</jats:p>
  • Access State: Open Access