Validation of oral brushing as a non‐invasive technique for the identification of feline oral squamous cell carcinoma by DNA methylation and TP53 mutation analysis
You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Validation of oral brushing as a non‐invasive technique for the identification of feline oral squamous cell carcinoma by DNA methylation and TP53 mutation analysis
Description:
<jats:title>Abstract</jats:title><jats:p>Feline oral squamous cell carcinoma (FOSCC) is a frequent and progressively invasive tumour. Early lesions are difficult to recognize based on the sole clinical examination and may be misinterpreted as non‐neoplastic. Mutations of <jats:italic>TP53</jats:italic> and epigenetic alterations of specific genes are present in FOSCC and may be early detected. Aim of this prospective study was to investigate the DNA methylation pattern of a 17‐gene panel and <jats:italic>TP53</jats:italic> mutational status of FOSCC cytological samples obtained by oral brushing. Results were compared with a control group, in order to validate this non‐invasive procedure for the screening of FOSCC. In FOSCC, the same analyses were carried out on the corresponding histological sample, if available. Thirty‐five FOSCC and 60 controls were included. Mutations of <jats:italic>TP53</jats:italic> were detected in 17 FOSCC brushings (48%) and in none of the controls (<jats:italic>P</jats:italic> < .001). Six genes (<jats:italic>ZAP70</jats:italic>, <jats:italic>FLI1</jats:italic>, <jats:italic>MiR124‐1</jats:italic>, <jats:italic>KIF1A</jats:italic>, <jats:italic>MAGEC2</jats:italic> and <jats:italic>MiR363</jats:italic>) were differentially methylated in FOSCC and were included in a methylation score. An algorithm based on <jats:italic>TP53</jats:italic> mutational status and methylation score allowed to differentiate FOSCC from controls with a 69% sensitivity and a 97% specificity (accuracy, 86%). In 19 FOSCC histological samples, <jats:italic>TP53</jats:italic> mutational status was fully concordant with brushings and a positive methylation score was observed in all cases. These results are promising for the identification of FOSCC by oral brushing, although some factors may limit the accuracy of this technique and further studies are required to assess its reproducibility in clinical practice.</jats:p>