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Medientyp:
Buch
Titel:
Plastids
:
methods and protocols
Enthält:
Primary endosymbiosis : emergence of the primary chloroplast and the chromatophore, two independent events
/ Eric Maréchal
Complex endosymbioses I : from primary to complex plastids, multiple independent events
/ Zoltán Füssy and Miroslav Oborník
Complex endosymbioses II : the nonphotosynthetic plastid of apicomplexa parasites (The apicoplast) and its integrated metabolism
/ Cyrille Y. Botté and Yoshiki Yamaryo-Botté
Diversity and plasticity of plastids in land plants
/ Katalin Solymosi, Johanna Lethin, and Henrik Aronsson
Main functions of plastids
/ Norbert Rolland, Imen Bouchnak, Lucas Moyet, Daniel Salvi, and Marcel Kuntz
Plastid transient and stable interactions with other cell compartments
/ Stefanie J. Mueller-Schuessele and Morgane Michaud
Imaging plastids in 2D and 3D : confocal and electron microscopy
/ Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F. Estrozi, Christine Moriscot, Guy Schoehn, Giovanni Finazzi, and Denis Falconet
Purification of chloroplasts and chloroplast subfractions : envelope, thylakoids, and stroma-from spinach, pea, and Arabidopsis thaliana
/ Maryse A. Block and Catherine Albrieux
Isolation of inner and outer membranes of the chloroplast envelope from spinach and pea
/ Maryse A. Block
Purification of nongreen plastids (proplastids and amyloplasts) from angiosperms, and isolation of their envelope membranes
/ Claude Alban and Etienne-Pascal Journet
In vitro protein import into isolated chloroplasts
/ Maryse A. Block
Analysis of the MTL supercomplex at contact sites between mitochondria and plastids
/ Morgane Michaud
Isolation of plastid fractions from the diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum
/ Alexander F. Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, and Carolina Río Bártulos
Isolating the Plasmodium falciparum apicoplast using magnetic beads
/ Cyrille Y. Botté, Geoffrey I. McFadden, and Yoshiki Yamaryo-Botté
Extraction and quantification of lipids from plant or algae
/ Valérie Gros, Josselin Lupette, and Juliette Jouhet
Quantitative assessment of the chloroplast lipidome
/ Valérie Gros and Juliette Jouhet
Determination of the DNA/RNA-associated subproteome from chloroplasts and other plastid types
/ Maha Chieb, Monique Liebers, Fabien Chevalier, Silva Lerbs-Mache, Robert Blanvillain, and Thomas Pfannschmidt
Mapping plastid transcript population by circular reverse transcription polymerase chain reaction
/ Florence Courtois and Livia Merendino
Bioinformatic analysis of chloroplast gene expression and RNA posttranscriptional maturations using RNA sequencing
/ Bastien Malbert, Guillem Rigaill, Veronique Brunaud, Claire Lurin, and Etienne Delannoy
Guide to the chloroplast transcriptome analysis using RNA-Seq
/ Elena J. S. Michel, Amber M. Hotto, Susan R. Strickler, David B. Stern, and Benoît Castandet
Toolkit for the characterization of the photoprotective capacity of green algae
/ M. Águila Ruiz-Sola and Dimitris Petroutsos
Rescue of deletion mutants to isolate plastid transformants in higher plants
/ Mohammad El Hajj, Mohd Fahdli Bin Hamdan, Elena Martin Avila, and Anil Day
Genetic analysis of chloroplast biogenesis, and function and mutant collections
/ Sho Fujii, Hajime Wada, and Koichi Kobayashi
Generation of mutants of nuclear-encoded plastid proteins using CRISPR/Cas9 in the diatom Phaeodactylum tricornutum
/ Guillaume Allorent, Erika Guglielmino, Cécile Giustini, and Florence Courtois
In Silico tools for the prediction of protein import into secondary plastids
/ Daniel Moog
AT Chloro: the first step when looking for information about subplastidial localization of proteins
/ Daniel Salvi, Sylvain Bournais, Lucas Moyet, Imen Bouchnak, Marcel Kuntz, Christophe Bruley, and Norbert Rolland.
Anmerkungen:
Includes bibliographical references and index
Beschreibung:
This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The chapters in this book also look at various techniques to analyze plastids through means of combining biology strategies from genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Plastids: Methods and Protocols is a valuable resource for students, engineers, and researchers who are interested in this evolving organelle and overall field