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Medientyp:
Buch
Titel:
Mesenchymal stem cells
:
methods and protocols
Enthält:
Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.]. Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger. A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino. Adipose-derived stem cells / John K. Fraser ... [et al.]. Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally. Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe. Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe. Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi. Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prockop. Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney
Beschreibung:
Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.]. Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger. A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino. Adipose-derived stem cells / John K. Fraser ... [et al.]. Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally. Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe. Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe. Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi. Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prockop. Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney