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Medientyp:
E-Artikel
Titel:
In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves
Beteiligte:
Schmelzer, Elmon;
Jahnen, Willi;
Hahlbrock, Klaus
Erschienen:
Proceedings of the National Academy of Sciences, 1988
Erschienen in:Proceedings of the National Academy of Sciences
Sprache:
Englisch
DOI:
10.1073/pnas.85.9.2989
ISSN:
0027-8424;
1091-6490
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>
Methods involving
<jats:italic>in situ</jats:italic>
RNA hybridization, immunohistochemistry, and microspectrophotometry of individual cells were used to localize the mRNA encoding chalcone synthase (the key enzyme of flavonoid biosynthesis), the enzyme protein, and the biosynthetic end products in cross sections of parsley leaves (
<jats:italic>Petroselinum crispum</jats:italic>
). The light-dependent, sequential occurrence of all three components was restricted to epidermal cells. The results are in agreement with the putative function of flavonoids (transcriptionally inducible, UV-protective pigments) and suggest that all biosynthetic steps occur in those cells in which the products accumulate.
</jats:p>