Paralysiella testudinis gen. nov., sp. nov., isolated from the cloaca of a toad-headed turtle (Mesoclemmys nasuta)

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Titel: Paralysiella testudinis gen. nov., sp. nov., isolated from the cloaca of a toad-headed turtle (Mesoclemmys nasuta)
Beteiligte: Busse, Hans-Jürgen; Kämpfer, Peter; Szostak, Michael Peter; Rückert, Christian; Spergser, Joachim
Erschienen: Microbiology Society, 2021
Erschienen in: International Journal of Systematic and Evolutionary Microbiology
Sprache: Englisch
DOI: 10.1099/ijsem.0.005114
ISSN: 1466-5026; 1466-5034
Schlagwörter: General Medicine ; Ecology, Evolution, Behavior and Systematics ; Microbiology
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Beschreibung: <jats:p>A bacterial strain designated 26B<jats:sup>T</jats:sup>, which had been isolated from the cloaca of a toad-headed turtle, was subjected to a comprehensive taxonomic study. Comparison of 16S rRNA gene sequences demonstrated that strain 26B<jats:sup>T</jats:sup> is a member of the family <jats:italic> <jats:named-content content-type="family"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1908" xlink:type="simple">Neisseriaceae</jats:ext-link> </jats:named-content> </jats:italic>. Based on highest similarity values, <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10234" xlink:type="simple">Neisseria animaloris</jats:ext-link> </jats:named-content> </jats:italic> DSM 21642<jats:sup>T</jats:sup> (95.15 %), <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1935" xlink:type="simple">Alysiella filiformis</jats:ext-link> </jats:named-content> </jats:italic> ATCC 15532<jats:sup>T</jats:sup> (95.06 %), <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.26595" xlink:type="simple">Uruburuella testudinis</jats:ext-link> </jats:named-content> </jats:italic> 07_OD624<jats:sup>T</jats:sup> (94.71 %), <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9352" xlink:type="simple">Uruburuella suis</jats:ext-link> </jats:named-content> </jats:italic> CCUG 47806<jats:sup>T</jats:sup> (94.66 %) and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9266" xlink:type="simple">Alysiella crassa</jats:ext-link> </jats:named-content> </jats:italic> DSM 2578<jats:sup>T</jats:sup> (94.64 %) were identified as the closest relatives. Average nucleotide identity values based on the <jats:sc>blast</jats:sc> algorithm (ANIb) indicated that <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9352" xlink:type="simple">U. suis</jats:ext-link> </jats:named-content> </jats:italic> (76.10/76.17 %), <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.20370" xlink:type="simple">Neisseria shayeganii</jats:ext-link> </jats:named-content> </jats:italic> 871<jats:sup>T</jats:sup> (74.34/74.51 %), <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13625" xlink:type="simple">Stenoxybacter acetivorans</jats:ext-link> </jats:named-content> </jats:italic> (73.30/73.41 %), <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10234" xlink:type="simple">N. animaloris</jats:ext-link> </jats:named-content> </jats:italic> (72.98/72.80) %, <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1935" xlink:type="simple">A. filiformis</jats:ext-link> </jats:named-content> </jats:italic> (71.14/71.21 %) and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9266" xlink:type="simple">A. crassa</jats:ext-link> </jats:named-content> </jats:italic> (70.53/71.15 %) are the next closest relatives. Like ANIb, genome-based phylogeny did not suggest the affiliation of strain 26B<jats:sup>T</jats:sup> with any established genus. The polyamine pattern consisted of the major compounds putrescine, 1,3-diaminopropane and spermidine and the major quinone was ubiquinone Q-8. In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an ornithine lipid were predominant. The fatty acid profile contained predominantly C<jats:sub>16 : 1</jats:sub> ω7<jats:italic>c</jats:italic>, C<jats:sub>12 : 0</jats:sub>, C<jats:sub>14 : 0</jats:sub>, C<jats:sub>16 : 0</jats:sub> and C<jats:sub>12 : 0</jats:sub> 3OH. The size of the genome was 2.91 Mbp and the genomic G+C content was 54.0 mol%. Since these data do not demonstrate an unambiguous association with any established genus, we here propose the novel genus <jats:italic>Paralysiella</jats:italic> with the type species <jats:italic>Paralysiella testudinis</jats:italic> gen. nov., sp. nov. The type strain is 26B<jats:sup>T</jats:sup> (=CCM 9137<jats:sup>T</jats:sup>=LMG 32212<jats:sup>T</jats:sup>).</jats:p>

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