Weiss-Hersh, Kathrin;
Garcia, Ada L.;
Marosvölgyi, Tamás;
Szklenár, Mónika;
Decsi, Tamás;
Rühl, Ralph
Saturated and monounsaturated fatty acids in membranes are determined by the gene expression of their metabolizing enzymes SCD1 and ELOVL6 regulated by the intake of dietary fat
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Medientyp:
E-Artikel
Titel:
Saturated and monounsaturated fatty acids in membranes are determined by the gene expression of their metabolizing enzymes SCD1 and ELOVL6 regulated by the intake of dietary fat
Beteiligte:
Weiss-Hersh, Kathrin;
Garcia, Ada L.;
Marosvölgyi, Tamás;
Szklenár, Mónika;
Decsi, Tamás;
Rühl, Ralph
Erschienen:
Springer Science and Business Media LLC, 2020
Beschreibung:
<jats:title>Abstract</jats:title><jats:sec><jats:title>Purpose</jats:title><jats:p>We investigated the effect of dietary fats on the incorporation of saturated (SAFAs) and monounsaturated dietary fatty acids (MUFAs) into plasma phospholipids and the regulation of the expression of lipid-metabolizing enzymes in the liver.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Mice were fed different diets containing commonly used dietary fats/oils (coconut fat, margarine, fish oil, sunflower oil, or olive oil) for 4 weeks (<jats:italic>n</jats:italic> = 6 per diet group). In a second experiment, mice (<jats:italic>n</jats:italic> = 6 per group) were treated for 7 days with synthetic ligands to activate specific nuclear hormone receptors (NHRs) and the hepatic gene expression of CYP26A1 was investigated. Hepatic gene expression of stearoyl-coenzyme A desaturase 1 (SCD1), elongase 6 (ELOVL6), and CYP26A1 was examined using quantitative real-time PCR (QRT-PCR). Fatty acid composition in mouse plasma phospholipids was analyzed by gas chromatography (GC).</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>We found significantly reduced hepatic gene expression of SCD1 and ELOVL6 after the fish oil diet compared with the other diets. This resulted in reduced enzyme-specific fatty acid ratios, e.g., 18:1n9/18:0 for SCD1 and 18:0/16:0 and 18:1n7/16:1n7 for ELOVL6 in plasma phospholipids. Furthermore, CYP26A1 a retinoic acid receptor-specific target was revealed as a new player mediating the suppressive effect of fish oil-supplemented diet on SCD1 and ELOVL6 hepatic gene expression.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Plasma levels of MUFAs and SAFAs strongly reflect an altered hepatic fatty acid-metabolizing enzyme expression after supplementation with different dietary fats/oils.</jats:p></jats:sec>