Next generation genetically encoded fluorescent sensors for serotonin

Medientyp: E-Artikel
Titel: Next generation genetically encoded fluorescent sensors for serotonin
Beteiligte: Kubitschke, Martin; Müller, Monika; Wallhorn, Lutz; Pulin, Mauro; Mittag, Manuel; Pollok, Stefan; Ziebarth, Tim; Bremshey, Svenja; Gerdey, Jill; Claussen, Kristin Carolin; Renken, Kim; Groß, Juliana; Gneiße, Pascal; Meyer, Niklas; Wiegert, J. Simon; Reiner, Andreas; Fuhrmann, Martin; Masseck, Olivia Andrea
Erschienen: Springer Science and Business Media LLC, 2022
Erschienen in: Nature Communications
Sprache: Englisch
DOI: 10.1038/s41467-022-35200-w
ISSN: 2041-1723
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Beschreibung: <jats:title>Abstract</jats:title><jats:p>We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.</jats:p>
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