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Beaume, M.; Köhler, T.; Greub, G.; Manuel, O.; Aubert, J-D.; Baerlocher, L.; Farinelli, L.; Buckling, A.; van Delden, C.; Achermann, Rita; Amico, Patrizia; Baumann, Philippe; Beldi, Guido; Benden, Christian; Berger, Christoph; Binet, Isabelle; Bochud, Pierre-Yves; Boely, Elsa; Bucher, Heiner; Bühler, Leo; Carell, Thierry; Catana, Emmanuelle; Chalandon, Yves; Geest, Sabina de; [...]

Rapid adaptation drives invasion of airway donor microbiota by Pseudomonas after lung transplantation

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  • Medientyp: E-Artikel
  • Titel: Rapid adaptation drives invasion of airway donor microbiota by Pseudomonas after lung transplantation
  • Beteiligte: Beaume, M.; Köhler, T.; Greub, G.; Manuel, O.; Aubert, J-D.; Baerlocher, L.; Farinelli, L.; Buckling, A.; van Delden, C.; Achermann, Rita; Amico, Patrizia; Baumann, Philippe; Beldi, Guido; Benden, Christian; Berger, Christoph; Binet, Isabelle; Bochud, Pierre-Yves; Boely, Elsa; Bucher, Heiner; Bühler, Leo; Carell, Thierry; Catana, Emmanuelle; Chalandon, Yves; Geest, Sabina de; [...]
  • Erschienen: Springer Science and Business Media LLC, 2017
  • Erschienen in: Scientific Reports
  • Sprache: Englisch
  • DOI: 10.1038/srep40309
  • ISSN: 2045-2322
  • Schlagwörter: Multidisciplinary
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>In cystic fibrosis (CF) patients, chronic airway infection by <jats:italic>Pseudomonas</jats:italic> leads to progressive lung destruction ultimately requiring lung transplantation (LT). Following LT, CF-adapted <jats:italic>Pseudomonas</jats:italic> strains, potentially originating from the sinuses, may seed the allograft leading to infections and reduced allograft survival. We investigated whether CF-adapted <jats:italic>Pseudomonas</jats:italic> populations invade the donor microbiota and adapt to the non-CF allograft. We collected sequential <jats:italic>Pseudomonas</jats:italic> isolates and airway samples from a CF-lung transplant recipient during two years, and followed the dynamics of the microbiota and <jats:italic>Pseudomonas</jats:italic> populations. We show that <jats:italic>Pseudomonas</jats:italic> invaded the host microbiota within three days post-LT, in association with a reduction in richness and diversity. A dominant mucoid and hypermutator <jats:italic>mutL</jats:italic> lineage was replaced after 11 days by non-mucoid strains. Despite antibiotic therapy, <jats:italic>Pseudomonas</jats:italic> dominated the allograft microbiota until day 95. We observed positive selection of pre-LT variants and the appearance of novel mutations. Phenotypic adaptation resulted in increased biofilm formation and swimming motility capacities. <jats:italic>Pseudomonas</jats:italic> was replaced after 95 days by a microbiota dominated by <jats:italic>Actinobacillus</jats:italic>. In conclusion, mucoid <jats:italic>Pseudomonas</jats:italic> adapted to the CF-lung remained able to invade the allograft. Selection of both pre-existing non-mucoid subpopulations and of novel phenotypic traits suggests rapid adaptation of <jats:italic>Pseudomonas</jats:italic> to the non-CF allograft.</jats:p>
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