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Nasrin, Shamima; Hegerle, Nicolas; Sen, Shaichi; Nkeze, Joseph; Sen, Sunil; Permala-Booth, Jasnehta; Choi, Myeongjin; Sinclair, James; Tapia, Milagritos D.; Johnson, J. Kristie; Sow, Samba O.; Thaden, Joshua T.; Fowler, Vance G.; Krogfelt, Karen A.; Brauner, Annelie; Protonotariou, Efthymia; Christaki, Eirini; Shindo, Yuichiro; Kwa, Andrea L.; Shakoor, Sadia; Singh-Moodley, Ashika; Perovic, Olga; Jacobs, Jan; Lunguya, Octavie; [...]

Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection

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  • Medientyp: E-Artikel
  • Titel: Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection
  • Beteiligte: Nasrin, Shamima; Hegerle, Nicolas; Sen, Shaichi; Nkeze, Joseph; Sen, Sunil; Permala-Booth, Jasnehta; Choi, Myeongjin; Sinclair, James; Tapia, Milagritos D.; Johnson, J. Kristie; Sow, Samba O.; Thaden, Joshua T.; Fowler, Vance G.; Krogfelt, Karen A.; Brauner, Annelie; Protonotariou, Efthymia; Christaki, Eirini; Shindo, Yuichiro; Kwa, Andrea L.; Shakoor, Sadia; Singh-Moodley, Ashika; Perovic, Olga; Jacobs, Jan; Lunguya, Octavie; [...]
  • Erschienen: Springer Science and Business Media LLC, 2022
  • Erschienen in: BMC Microbiology
  • Sprache: Englisch
  • DOI: 10.1186/s12866-021-02427-4
  • ISSN: 1471-2180
  • Schlagwörter: Microbiology (medical) ; Microbiology
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p><jats:italic>Pseudomonas aeruginosa</jats:italic> is an opportunistic pathogen that causes a wide range of acute and chronic infections and is frequently associated with healthcare-associated infections. Because of its ability to rapidly acquire resistance to antibiotics, <jats:italic>P. aeruginosa</jats:italic> infections are difficult to treat. Alternative strategies, such as a vaccine, are needed to prevent infections<jats:italic>.</jats:italic> We collected a total of 413 <jats:italic>P. aeruginosa</jats:italic> isolates from the blood and cerebrospinal fluid of patients from 10 countries located on 4 continents during 2005–2017 and characterized these isolates to inform vaccine development efforts. We determined the diversity and distribution of O antigen and flagellin types and antibiotic susceptibility of the invasive <jats:italic>P. aeruginosa</jats:italic>. We used an antibody-based agglutination assay and PCR for O antigen typing and PCR for flagellin typing. We determined antibiotic susceptibility using the Kirby-Bauer disk diffusion method.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Of the 413 isolates, 314 (95%) were typed by an antibody-based agglutination assay or PCR (<jats:italic>n</jats:italic> = 99). Among the 20 serotypes of <jats:italic>P. aeruginosa</jats:italic>, the most common serotypes were O1, O2, O3, O4, O5, O6, O8, O9, O10 and O11; a vaccine that targets these 10 serotypes would confer protection against more than 80% of invasive <jats:italic>P. aeruginosa</jats:italic> infections. The most common flagellin type among 386 isolates was FlaB (41%). Resistance to aztreonam (56%) was most common, followed by levofloxacin (42%). We also found that 22% of strains were non-susceptible to meropenem and piperacillin-tazobactam. Ninety-nine (27%) of our collected isolates were resistant to multiple antibiotics. Isolates with FlaA2 flagellin were more commonly multidrug resistant (<jats:italic>p</jats:italic> = 0.04).</jats:p> </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> <jats:p>Vaccines targeting common O antigens and two flagellin antigens, FlaB and FlaA2, would offer an excellent strategy to prevent <jats:italic>P. aeruginosa</jats:italic> invasive infections.</jats:p> </jats:sec>
  • Zugangsstatus: Freier Zugang