Ollero, Juan Antonio Miralles De Imperial;
Ortega, Alejandro Gallego;
Muñoz, María Norte;
Di Pierdomenico, Johnny;
Valiente‐Soriano, Francisco Javier;
Vidal‐Sanz, Manuel
Microglial activation and retinal pigment epithelium alteration in a model of focal LED‐induced phototoxicity. Neuroprotection afforded by bFGF and minocycline
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Medientyp:
E-Artikel
Titel:
Microglial activation and retinal pigment epithelium alteration in a model of focal LED‐induced phototoxicity. Neuroprotection afforded by bFGF and minocycline
Beteiligte:
Ollero, Juan Antonio Miralles De Imperial;
Ortega, Alejandro Gallego;
Muñoz, María Norte;
Di Pierdomenico, Johnny;
Valiente‐Soriano, Francisco Javier;
Vidal‐Sanz, Manuel
Beschreibung:
<jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p><jats:bold>Purpose:</jats:bold> To study the microglial reaction and degeneration of the retinal pigment epithelium (RPE) and its protection with basic fibroblast growth factor (bFGF) administered alone or combined with minocycline (MC) in a model of focal light‐emitting diode (LED)‐induced phototoxicity (LIP) in mice.</jats:p><jats:p><jats:bold>Methods:</jats:bold> In dark‐adapted adult C57 mice (20–25 g), the left eye was dilated and exposed for 45 seconds to a blue LED (400 nm; 500 lux) placed 2 mm perpendicular to the corneal apex (<jats:italic>n</jats:italic> = 71). Mice were treated with bFGF (0.5 μg) administered alone or in combination with MC (45 mg/kg). bFGF was administered in a single intravitreal injection just after LIP and the MC was daily injected intraperitoneally, starting the day before LIP (<jats:italic>n</jats:italic> = 10–13 per group). Vehicle or naïve groups were used as controls (<jats:italic>n</jats:italic> = 6–10 each group). Retinas were immunodetected with anti‐arrestin and anti‐Iba1 antibodies to study cone outer segments (a<jats:sup>+</jats:sup>OS) and microglial cells (Iba1<jats:sup>+</jats:sup>cells) and RPEs were immunodetected with anti‐ZO1 to study RPE cells, at 3 and 7 days after LIP. The number of cones and microglial cells was studied within a pre‐fixed area (PFA) of 0.9 mm diameter at the centre of the lesion.</jats:p><jats:p><jats:bold>Results:</jats:bold> Treatment with bFGF alone or in combination with MC was effective in protecting a<jats:sup>+</jats:sup>OS within the PFA compared to vehicles at 7 days after LIP (<jats:italic>p</jats:italic> = 0.034 and <jats:italic>p</jats:italic> = 0.001, respectively). The combined treatment of bFGF and MC was effective in reducing the number of Iba1<jats:sup>+</jats:sup>cells within the PFA in the outer plexiform layer at 7 days after LIP (<jats:italic>p</jats:italic> = 0.0003). However, the treatments had no restorative effect on RPE cell survival or morphological integrity at 7 days after LIP (<jats:italic>p</jats:italic> = 0.6762).</jats:p><jats:p><jats:bold>Conclusions:</jats:bold> Administration of bFGF alone or in combination with MC increased a<jats:sup>+</jats:sup>OS survival and reduces Iba1<jats:sup>+</jats:sup>cell activation but does not reduce RPE damage.</jats:p></jats:sec>