Beschreibung:
<jats:title>Summary</jats:title><jats:p>We determined the genome‐wide environmental stress response (ESR) expression profile of <jats:italic>Candida glabrata</jats:italic>, a human pathogen related to <jats:italic>Saccharomyces cerevisiae</jats:italic>. Despite different habitats, <jats:italic>C. glabrata</jats:italic>, <jats:italic>S. cerevisiae</jats:italic>, <jats:italic>Schizosaccharomyces pombe</jats:italic> and <jats:italic>Candida albicans</jats:italic> have a qualitatively similar ESR. We investigate the function of the <jats:italic>C. glabrata</jats:italic> syntenic orthologues to the ESR transcription factor Msn2. The <jats:italic>C. glabrata</jats:italic> orthologues CgMsn2 and CgMsn4 contain a motif previously referred to as HD1 (homology domain 1) also present in Msn2 orthologues from fungi closely related to <jats:italic>S. cerevisiae</jats:italic>. We show that regions including this motif confer stress‐regulated intracellular localization when expressed in <jats:italic>S. cerevisiae</jats:italic>. Site‐directed mutagenesis confirms that nuclear export of CgMsn2 in <jats:italic>C. glabrata</jats:italic> requires an intact HD1. Transcript profiles of CgMsn2/4 mutants and CgMsn2 overexpression strains show that they regulate a part of the CgESR. CgMsn2 complements a <jats:italic>S</jats:italic>. <jats:italic>cerevisiae msn2</jats:italic> null mutant and in stressed <jats:italic>C. glabrata</jats:italic> cells, rapidly translocates from the cytosol to the nucleus. CgMsn2 is required for full resistance against severe osmotic stress and rapid and full induction of trehalose synthesis genes (<jats:italic>TPS1</jats:italic>, <jats:italic>TPS2</jats:italic>). Constitutive activation of CgMsn2 is detrimental for <jats:italic>C. glabrata.</jats:italic> These results establish an Msn2‐regulated general stress response in <jats:italic>C. glabrata</jats:italic>.</jats:p>