Beschreibung:
<jats:title>ABSTRACT</jats:title>
<jats:p>Formylpeptide chemoattractant binds specifically to leukocyte cell surface receptors and during chemotactic activation, is internalized and processed by these cells. Using electron microscope autoradiographic techniques, we have examined the binding, uptake and disposition of fNle-Leu-Phe-Nle-[125I]Tyr-Lys by rabbit peritoneal neutrophils. Cells were incubated with ligand for 15 min at 4°C, rinsed and then further incubated in buffer at 4°C, 15°C, 24°C, or 37°C for 0–40min. For all cells incubated at 4°C, grains were predominantly localized on the plasma membrane. Initially, this formylpeptide was seen in small clusters or microaggregates that were not associated with coated pits. Upon further incubation at 37°C for 1 min, formylpeptide clustering on the plasma membrane increased and extensive internalization of formylpeptide was observed. Endocytosis of formylpeptide-receptor complexes clearly involved uncoated membrane pits and vesicles but did not appear to involve coated pits or coated vesicles. In the following 3 min, peptide proceeded in waves through compartments composed of small uncoated endocytic vesicles, then large vesicles, and then into dense granules. After 4 min at 37°C, the most active phase of intracellular processing subsided. The percentage of grains in the cytoplasm, granule and small vesicle compartments very gradually increased during the remainder of the 40 min incubation. Formylpeptide neither bound at 4 °C nor accumulated at 37 °C on the cell surface in proximity to the underlying Golgi/centrosome region of the cell. At 24°C, processing was slowed but formylpeptide-receptor complexes proceeded through a similar series of compartments. The q for formylpeptide uptake at 37 °C was 15-20 s, whereas at 24°C the q was approximately 10min. No uptake was observed at 15°C. The distinctive characteristics of formylpeptide binding and receptor-complex uptake seen here may be essential in initiating and maintaining continued chemotactic responsiveness.</jats:p>