Chavhan, S. G.;
Balachandran, C.;
Nambi, A. P.;
Raj, G. Dhinakar;
Vairamuthu, S.
Assessment of Anti-Carcinogenic Potential of Neem (Azadirachta indica) Leaf Extract Loaded Calcium Phosphate Nanoparticles against Experimentally Induced Mammary Carcinogenesis in Rats
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Medientyp:
E-Artikel
Titel:
Assessment of Anti-Carcinogenic Potential of Neem (Azadirachta indica) Leaf Extract Loaded Calcium Phosphate Nanoparticles against Experimentally Induced Mammary Carcinogenesis in Rats
Beteiligte:
Chavhan, S. G.;
Balachandran, C.;
Nambi, A. P.;
Raj, G. Dhinakar;
Vairamuthu, S.
Beschreibung:
<jats:p>Considering the need for alternative medicine in alleviation of tumors and use of nanotechnology in furthering the action of herbal/natural products, the present study was designed to evaluate the anti-carcinogenic potential of Neem leaf Extract (NE) loaded calcium phosphate nanoparticles (NE-CaNP) on 7, 12-Dimethylbenzanthracene (DMBA) induced mammary tumors in female Sprague-Dawley rats. Ultrastructurally, the NE-CaNP were smooth, spherical with a tendency to agglomerate and evenly distributed. The NE-CaNP had a mean diameter of 231.4 ± 89.2 nm and zeta potential of -31.3mV. The mean coupling efficiency of CaNP was 90-91 %. The experimental trial consisted of control, NE-CaNP control, DMBA, DMBA+NE and DMBA+NE-CaNP groups. The mean latency periods for occurrence of mammary tumor were significantly (P ≤ 0.05) increased in the DMBA+NE and DMBA+NE-CaNP groups compared to the DMBA group. The mean latency period in the DMBA+NE-CaNP group was significantly (P ≤ 0.05) higher than the DMBA+NE group. The mean tumor frequency, volume and weight were significantly (P ≤ 0.05) decreased in the DMBA+NE-CaNP group. Histopathologically, the number of benign lesions was found highest (47.54%) in DMBA+NE-CaNP group rats. The relative percent reduction in malignancy as compared to the DMBA group was 42.86% and 54.29% in the DMBA+NE and DMBA+NE-CaNP groups respectively. In conclusion, the neem leaf extract loaded calcium phosphate nanoparticles were found to have better anti-carcinogenic potential by significantly reducing the incidence, frequency, weight, volume, malignancy and increased the tumor latency period of DMBA induced mammary tumors in female Sprague-Dawley rats as compared to rats treated with neem extract alone. Findings of the present study suggested that the neem leaf extract loaded calcium phosphate nanoparticles (NE-CaNP) has immense anticancer potential in terms of reduction in tumor burden and malignancy.</jats:p>