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Schmutzler, Oliver; Graf, Sebastian; Behm, Nils; Mansour, Wael Y.; Blumendorf, Florian; Staufer, Theresa; Körnig, Christian; Salah, Dina; Kang, Yanan; Peters, Jan N.; Liu, Yang; Feliu, Neus; Parak, Wolfgang J.; Burkhardt, Anja; Gargioni, Elisabetta; Gennis, Sabrina; Chandralingam, Sharah; Höeg, Finn; Maison, Wolfgang; Rothkamm, Kai; Schulz, Florian; Grüner, Florian

X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples

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  • Medientyp: E-Artikel
  • Titel: X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples
  • Beteiligte: Schmutzler, Oliver; Graf, Sebastian; Behm, Nils; Mansour, Wael Y.; Blumendorf, Florian; Staufer, Theresa; Körnig, Christian; Salah, Dina; Kang, Yanan; Peters, Jan N.; Liu, Yang; Feliu, Neus; Parak, Wolfgang J.; Burkhardt, Anja; Gargioni, Elisabetta; Gennis, Sabrina; Chandralingam, Sharah; Höeg, Finn; Maison, Wolfgang; Rothkamm, Kai; Schulz, Florian; Grüner, Florian
  • Erschienen: MDPI AG, 2021
  • Erschienen in: International Journal of Molecular Sciences
  • Sprache: Englisch
  • DOI: 10.3390/ijms22073691
  • ISSN: 1422-0067
  • Schlagwörter: Inorganic Chemistry ; Organic Chemistry ; Physical and Theoretical Chemistry ; Computer Science Applications ; Spectroscopy ; Molecular Biology ; General Medicine ; Catalysis
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.</jats:p>
  • Zugangsstatus: Freier Zugang