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Medientyp:
E-Artikel
Titel:
Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways
Beteiligte:
Kramer, Markus M.;
Lataster, Levin;
Weber, Wilfried;
Radziwill, Gerald
Erschienen:
MDPI AG, 2021
Erschienen in:International Journal of Molecular Sciences
Sprache:
Englisch
DOI:
10.3390/ijms22105300
ISSN:
1422-0067
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>Biological signals are sensed by their respective receptors and are transduced and processed by a sophisticated intracellular signaling network leading to a signal-specific cellular response. Thereby, the response to the signal depends on the strength, the frequency, and the duration of the stimulus as well as on the subcellular signal progression. Optogenetic tools are based on genetically encoded light-sensing proteins facilitating the precise spatiotemporal control of signal transduction pathways and cell fate decisions in the absence of natural ligands. In this review, we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins. We briefly discuss the most common optogenetic switches and their mode of action. The main part deals with the engineering and application of optogenetic tools for the control of transmembrane receptors including receptor tyrosine kinases, the T cell receptor and integrins, and their effector proteins. We also address the hallmarks of optogenetics, the spatial and temporal control of signaling events.</jats:p>